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RBM41 Polyclonal antibody

RBM41 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15784-1-AP

Synonyms

bB383K5.1, RBM41, RNA binding motif protein 41, RNA binding protein 41

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RBM41 Polyclonal antibody (15784-1-AP)

    WB analysis using 15784-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15784-1-AP (RBM41 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using RBM41 Polyclonal antibody (15784-1-AP)

    WB analysis of A549 using 15784-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 15784-1-AP (RBM41 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SGC-7901 cells using RBM41 Polyclonal antibody (15784-1-AP)

    WB analysis of SGC-7901 using 15784-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 15784-1-AP (RBM41 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using RBM41 Polyclonal antibody (15784-1-AP)

    IHC staining of human endometrial cancer using 15784-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 15784-1-AP (RBM41 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using RBM41 Polyclonal antibody (15784-1-AP)

    IHC staining of human endometrial cancer using 15784-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 15784-1-AP (RBM41 antibody) at dilution of 1:100 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RBM41 Polyclonal antibody (15784-1-AP)

    IF Staining of HepG2 using 15784-1-AP

    Immunofluorescent analysis of HepG2 cells, using RBM41 antibody 15784-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inHeLa cells, A549 cells, SGC-7901 cells, HepG2 cells
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15784-1-AP targets RBM41 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBM41 fusion protein Ag8483
Full Name RNA binding motif protein 41
Calculated Molecular Weight 413 aa, 47 kDa
Observed Molecular Weight 43-47 kDa
GenBank Accession NumberBC006986
Gene Symbol RBM41
Gene ID (NCBI) 55285
RRIDAB_2176112
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RBM41 antibody 15784-1-APDownload protocol
IHC protocol for RBM41 antibody 15784-1-APDownload protocol
IF protocol for RBM41 antibody 15784-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols