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RBM28 Polyclonal antibody

RBM28 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16484-1-AP

Synonyms

RNA-binding protein 28, RNA-binding motif protein 28, RNA binding protein 28, RNA binding motif protein 28

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using RBM28 Polyclonal antibody (16484-1-AP)

    WB analysis of NIH/3T3 using 16484-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16484-1-AP (RBM28 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-251 cells using RBM28 Polyclonal antibody (16484-1-AP)

    WB analysis of U-251 using 16484-1-AP

    WB result of RBM28 antibody (16484-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM28 transfected U-251 cells.

  • Immunoprecipitation (IP) experiment of NIH/3T3 cells using RBM28 Polyclonal antibody (16484-1-AP)

    IP experiment of NIH/3T3 using 16484-1-AP

    IP result of anti-RBM28 (IP:16484-1-AP, 4ug; Detection:16484-1-AP 1:1000) with NIH/3T3 cells lysate 1120 ug.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using RBM28 Polyclonal antibody (16484-1-AP)

    IF Staining of NIH/3T3 using 16484-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 16484-1-AP (RBM28 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inNIH/3T3 cells, U-251 cells
Positive IP detected inNIH/3T3 cells
Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16484-1-AP targets RBM28 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBM28 fusion protein Ag9606
Full Name RNA binding motif protein 28
Calculated Molecular Weight 756 aa, 86 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC013889
Gene Symbol RBM28
Gene ID (NCBI) 55131
RRIDAB_2238116
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RBM28 antibody 16484-1-APDownload protocol
IF protocol for RBM28 antibody 16484-1-APDownload protocol
IP protocol for RBM28 antibody 16484-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

J Biol Chem

RNA binding protein RBM28 can translocate from the nucleolus to the nucleoplasm to inhibit the transcriptional activity of p53.

Authors - Xin Lin
View Article
humanWB

Cancer Lett

RNA-binding motif protein 28 enhances angiogenesis by improving STAT3 translation in hepatocellular carcinoma

Authors - Hexu Han
View Article