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RBM16 Polyclonal antibody

RBM16 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 18893-1-AP

Synonyms

SCAF8, RNA-binding motif protein 16, RNA binding protein 16, KIAA1116, CDC5L complex-associated protein 7

Validation Data Gallery

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  • Western Blot (WB) analysis of HT-1080 cells using RBM16 Polyclonal antibody (18893-1-AP)

    WB analysis of HT-1080 using 18893-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RBM16 Polyclonal antibody (18893-1-AP)

    WB analysis using 18893-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RBM16 Polyclonal antibody (18893-1-AP)

    WB analysis of HeLa using 18893-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using RBM16 Polyclonal antibody (18893-1-AP)

    WB analysis of NIH/3T3 using 18893-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using RBM16 Polyclonal antibody (18893-1-AP)

    WB analysis of A549 using 18893-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using RBM16 Polyclonal antibody (18893-1-AP)

    WB analysis of L02 using 18893-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using RBM16 Polyclonal antibody (18893-1-AP)

    IP experiment of HeLa using 18893-1-AP

    IP result of anti-RBM16 (IP:18893-1-AP, 4ug; Detection:18893-1-AP 1:800) with HeLa cells lysate 3600ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using RBM16 Polyclonal antibody (18893-1-AP)

    IHC staining of human testis using 18893-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18893-1-AP (RBM16 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using RBM16 Polyclonal antibody (18893-1-AP)

    IHC staining of human testis using 18893-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18893-1-AP (RBM16 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using RBM16 Polyclonal antibody (18893-1-AP)

    IHC staining of human cervical cancer using 18893-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 18893-1-AP (RBM16 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using RBM16 Polyclonal antibody (18893-1-AP)

    IHC staining of human cervical cancer using 18893-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 18893-1-AP (RBM16 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using RBM16 Polyclonal antibody (18893-1-AP)

    IHC staining of human cervical cancer using 18893-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 18893-1-AP (RBM16 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using RBM16 Polyclonal antibody (18893-1-AP)

    IHC staining of human cervical cancer using 18893-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 18893-1-AP (RBM16 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RBM16 Polyclonal antibody (18893-1-AP)

    IF Staining of HeLa using 18893-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM16 antibody (18893-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RBM16 Polyclonal antibody (18893-1-AP)

    IF Staining of HepG2 using 18893-1-AP

    Immunofluorescent analysis of HepG2 cells, using RBM16 antibody 18893-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inA549 cells, HeLa cells, NIH/3T3 cells, L02 cells, HT-1080 cells, THP-1 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman testis tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18893-1-AP targets RBM16 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBM16 fusion protein Ag13499
Full Name RNA binding motif protein 16
Calculated Molecular Weight 1271 aa, 141 kDa
Observed Molecular Weight 170 kDa
GenBank Accession NumberBC070071
Gene Symbol RBM16
Gene ID (NCBI) 22828
RRIDAB_2175908
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBM16, also named as SCAF8, CCAP7, KIAA1116, is a 1271 amino acid protein, which interacts with POLR2A. RBM16 may play a role in mRNA processing. Immunoblot analysis of the total rat liver nuclear and nuclear matrix protein fractions revealed a single band with an apparent molecular mass of 170 kDa. While the apparent molecular mass is slightly larger than the 140 kDa predicted from the cDNA sequence, we note that the protein is rich in proline residues (10%) and therefore may display aberrant mobility in SDS gel electrophoresis. (PMID: 9528809 )

Protocols

Product Specific Protocols
WB protocol for RBM16 antibody 18893-1-APDownload protocol
IHC protocol for RBM16 antibody 18893-1-APDownload protocol
IF protocol for RBM16 antibody 18893-1-APDownload protocol
IP protocol for RBM16 antibody 18893-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols