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  • Western Blot (WB) analysis of various lysates using RBM12B Polyclonal antibody (17137-1-AP)

    WB analysis using 17137-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17137-1-AP (RBM12B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using RBM12B Polyclonal antibody (17137-1-AP)

    IHC staining of human liver using 17137-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17137-1-AP (RBM12B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using RBM12B Polyclonal antibody (17137-1-AP)

    IHC staining of human liver using 17137-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17137-1-AP (RBM12B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using RBM12B Polyclonal antibody (17137-1-AP)

    IF Staining of A431 using 17137-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RBM12B antibody (17137-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

RBM12B Polyclonal antibody

RBM12B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 17137-1-AP
Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

RBM12B, RNA binding motif protein 12B, RNA binding protein 12B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inA431 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

17137-1-AP targets RBM12B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBM12B fusion protein Ag10894 Predict reactive species
Full Name RNA binding motif protein 12B
Calculated Molecular Weight 765 aa, 87 kDa, 118 kDa
Observed Molecular Weight118-130 kDa
GenBank Accession NumberBC039260
Gene Symbol RBM12B
Gene ID (NCBI) 389677
RRIDAB_2175746
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IXT5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RBM12B antibody 17137-1-APDownload protocol
IHC protocol for RBM12B antibody 17137-1-APDownload protocol
IF protocol for RBM12B antibody 17137-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Biol

Stress granules regulate stress-induced paraspeckle assembly.

Authors - Haiyan An
View Article