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RAP2B Polyclonal antibody

RAP2B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16266-1-AP

Synonyms

Ras-related protein Rap-2b, Ras related protein Rap 2b, EC:3.6.5.2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAP2B Polyclonal antibody (16266-1-AP)

    WB analysis using 16266-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16266-1-AP (RAP2B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using RAP2B Polyclonal antibody (16266-1-AP)

    WB analysis of rat brain using 16266-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 16266-1-AP (RAP2B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using RAP2B Polyclonal antibody (16266-1-AP)

    WB analysis of MCF-7 using 16266-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 16266-1-AP (RAP2B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RAP2B Polyclonal antibody (16266-1-AP)

    WB analysis of HEK-293 using 16266-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16266-1-AP (RAP2B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using RAP2B Polyclonal antibody (16266-1-AP)

    IHC staining of human stomach using 16266-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16266-1-AP (RAP2B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse brain tissue, MCF-7 cells, rat brain tissue, HEK-293 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16266-1-AP targets RAP2B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAP2B fusion protein Ag9331
Full Name RAP2B, member of RAS oncogene family
Calculated Molecular Weight 183 aa, 21 kDa
Observed Molecular Weight 19-22 kDa
GenBank Accession NumberBC012362
Gene Symbol RAP2B
Gene ID (NCBI) 5912
RRIDAB_2177311
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAP2B belongs to a family of RAS-related genes. It share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. Rap2B is a platelet protein that is activated by thrombin and involved in platelet activation. RAP2B may be a novel candidate oncogene that plays important roles in carcinogenesis through activation of NF-kappaB pathway.

Protocols

Product Specific Protocols
WB protocol for RAP2B antibody 16266-1-APDownload protocol
IHC protocol for RAP2B antibody 16266-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Biol Chem

DHHC5 protein palmitoylates flotillin-2 and is rapidly degraded on induction of neuronal differentiation in cultured cells.

Authors - Li Yi Y
View Article