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RAP1GAP Polyclonal antibody

RAP1GAP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19174-1-AP

Synonyms

RAPGAP, RAP1GAP1, RAP1GA1, Rap1 GTPase-activating protein 1, RAP1 GTPase activating protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAP1GAP Polyclonal antibody (19174-1-AP)

    WB analysis using 19174-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19174-1-AP (RAP1GAP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RAP1GAP Polyclonal antibody (19174-1-AP)

    WB analysis of HeLa using 19174-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19174-1-AP (RAP1GAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using RAP1GAP Polyclonal antibody (19174-1-AP)

    WB analysis of Jurkat using 19174-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19174-1-AP (RAP1GAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using RAP1GAP Polyclonal antibody (19174-1-AP)

    IHC staining of human ovary cancer using 19174-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 19174-1-AP (RAP1GAP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAP1GAP Polyclonal antibody (19174-1-AP)

    IF Staining of HeLa using 19174-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RAP1GAP antibody (19174-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19174-1-AP targets RAP1GAP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAP1GAP fusion protein Ag5576
Full Name RAP1 GTPase activating protein
Calculated Molecular Weight 73 kDa
Observed Molecular Weight 85-95 kDa
GenBank Accession NumberBC054490
Gene Symbol RAP1GAP
Gene ID (NCBI) 5909
RRIDAB_2177291
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RAP1GAP antibody 19174-1-APDownload protocol
IHC protocol for RAP1GAP antibody 19174-1-APDownload protocol
IF protocol for RAP1GAP antibody 19174-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols