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  • Featured Product
  • KD/KO Validated

RAD21 Polyclonal antibody

RAD21 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, canine

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27071-1-AP

Synonyms

hHR21, HR21, HRAD21, KIAA0078, MCD1, Nuclear matrix protein 1, NXP 1, NXP1, RAD21, RAD21 homolog (S. pombe), SCC1, SCC1 homolog

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using RAD21 Polyclonal antibody (27071-1-AP)

    WB analysis of A549 using 27071-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 27071-1-AP (RAD21 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RAD21 Polyclonal antibody (27071-1-AP)

    WB analysis of HeLa using 27071-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 27071-1-AP (RAD21 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RAD21 Polyclonal antibody (27071-1-AP)

    IHC staining of human breast cancer using 27071-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27071-1-AP (RAD21 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RAD21 Polyclonal antibody (27071-1-AP)

    IHC staining of human breast cancer using 27071-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27071-1-AP (RAD21 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using RAD21 Polyclonal antibody (27071-1-AP)

    IF Staining of MDCK using 27071-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using RAD21 antibody (27071-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using RAD21 Polyclonal antibody (27071-1-AP)

    IF Staining of MDCK using 27071-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using RAD21 antibody (27071-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inA549 cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMDCK cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27071-1-AP targets RAD21 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, canine samples.

Tested Reactivity human, canine
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAD21 fusion protein Ag25481
Full Name RAD21 homolog (S. pombe)
Calculated Molecular Weight 631 aa, 72 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC050381
Gene Symbol RAD21
Gene ID (NCBI) 5885
RRIDAB_2880742
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAD21, also named as Nuclear matrix protein 1, is a 631 amino acid protein, which belongs to the rad21 family. The calculated molecular weight of RAD21 is 72 kDa, but the phosphorylated RAD21 is about 120 kDa. The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells

Protocols

Product Specific Protocols
WB protocol for RAD21 antibody 27071-1-APDownload protocol
IHC protocol for RAD21 antibody 27071-1-APDownload protocol
IF protocol for RAD21 antibody 27071-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Lett

IQGAP3 interacts with Rad17 to recruit the Mre11-Rad50-Nbs1 complex and contributes to radioresistance in lung cancer.

Authors - Yulan Zeng
View Article
humanIHC,WB

Front Oncol

RAD21 Confers Poor Prognosis and Affects Ovarian Cancer Sensitivity to Poly(ADP-Ribose)Polymerase Inhibitors Through DNA Damage Repair.

Authors - Rui Gou
  • KD Validated
View Article
humanWB

Cell Death Dis

EGFR-ERK induced activation of GRHL1 promotes cell cycle progression by up-regulating cell cycle related genes in lung cancer.

Authors - Yiming He
View Article