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RAC2 Polyclonal antibody

RAC2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 20573-1-AP

Synonyms

Small G protein, Ras-related C3 botulinum toxin substrate 2, p21-Rac2, p21 Rac2, HSPC022

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using RAC2 Polyclonal antibody (20573-1-AP)

    WB analysis of Jurkat using 20573-1-AP

    WB result of RAC2 antibody (20573-1-AP, 1:600) with si-control and si-RAC2 transfected Jurkat cells..

  • Western Blot (WB) analysis of Jurkat cells using RAC2 Polyclonal antibody (20573-1-AP)

    WB analysis of Jurkat using 20573-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20573-1-AP (RAC2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using RAC2 Polyclonal antibody (20573-1-AP)

    WB analysis of HL-60 using 20573-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 20573-1-AP (RAC2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MOLT-4 cells using RAC2 Polyclonal antibody (20573-1-AP)

    IP experiment of MOLT-4 using 20573-1-AP

    IP result of anti-RAC2 (IP:20573-1-AP, 4ug; Detection:20573-1-AP 1:500) with MOLT-4 cells lysate 1800 ug.

Tested Applications

Positive WB detected inJurkat cells, HL-60 cells
Positive IP detected inMOLT-4 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20573-1-AP targets RAC2 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2)
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberNM_002872
Gene Symbol RAC2
Gene ID (NCBI) 5880
RRIDAB_10694419
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAC2, also named as GX, belongs to the small GTPase superfamily and Rho family. RAC2 is a plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. RAC2 augments the production of reactive oxygen species (ROS) by NADPH oxidase. Defects in RAC2 are the cause of neutrophil immunodeficiency syndrome. The antibody is specific to RAC2.

Protocols

Product Specific Protocols
WB protocol for RAC2 antibody 20573-1-APDownload protocol
IP protocol for RAC2 antibody 20573-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols