human lung tissue were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
human lung tissue were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis using 11916-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11916-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
human brain tissue were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11916-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 11916-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse lung using 11916-1-AP
IP result of anti-RABL3 (IP:11916-1-AP, 3ug; Detection:11916-1-AP 1:300) with mouse lung tissue lysate 4000ug.
IP result of anti-RABL3 (IP:11916-1-AP, 3ug; Detection:11916-1-AP 1:300) with mouse lung tissue lysate 4000ug.
IHC staining of human breast cancer using 11916-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11916-1-AP (RABL3 Antibody) at dilution of 1:50 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11916-1-AP (RABL3 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human breast cancer using 11916-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11916-1-AP (RABL3 Antibody) at dilution of 1:50 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11916-1-AP (RABL3 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver cancer using 11916-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11916-1-AP (RABL3 Antibody) at dilution of 1:50 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11916-1-AP (RABL3 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver cancer using 11916-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11916-1-AP (RABL3 Antibody) at dilution of 1:50 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11916-1-AP (RABL3 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human breast cancer using 11916-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human kidney using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of A549 using 11916-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using RABL3 antibody (11916-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using RABL3 antibody (11916-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
FC experiment of HEK-293 using 11916-1-AP
1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human RABL3 (11916-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human RABL3 (11916-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
HEK-293 cells, mouse brain tissue, human brain tissue, human lung tissue, HUVEC cells, U-251 cells
Positive IP detected in
mouse lung tissue
Positive IHC detected in
human breast cancer tissue, human liver cancer tissue, human cervical cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
A549 cells
Positive FC (Intra) detected in
HEK-293 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:5000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
The gene of Rabl3 is located on 3q13.3 and is composed of eight exons and seven introns. It is a member of the Rab subfamily, the largest group in the Ras superfamily of small guanosine triphosphatases (GTPases).
High Expression of Rab-like 3 (Rabl3) is Associated with Poor Survival of Patients with Non-Small Cell Lung Cancer via Repression of MAPK8/9/10-Mediated Autophagy.
human lung tissue were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis using 11916-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11916-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11916-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 11916-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human breast cancer using 11916-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11916-1-AP (RABL3 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human breast cancer using 11916-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11916-1-AP (RABL3 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver cancer using 11916-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11916-1-AP (RABL3 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver cancer using 11916-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11916-1-AP (RABL3 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human breast cancer using 11916-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human breast cancer using 11916-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human cervical cancer using 11916-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human cervical cancer using 11916-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 11916-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 11916-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 11916-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 11916-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 40x lens).
IP Figures
IP experiment of mouse lung using 11916-1-AP
IP result of anti-RABL3 (IP:11916-1-AP, 3ug; Detection:11916-1-AP 1:300) with mouse lung tissue lysate 4000ug.
IF/ICC Figures
IF Staining of A549 using 11916-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using RABL3 antibody (11916-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
FC (INTRA) Figures
FC experiment of HEK-293 using 11916-1-AP
1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human RABL3 (11916-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
RABL3 Polyclonal antibody
Catalog Number
11916-1-AP
Citations
2
Dilutions
WB : 1:1000-1:5000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:200-1:800 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Reactivity
human, mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
