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RABL2B Polyclonal antibody

RABL2B Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 11588-1-AP

Synonyms

Rab like protein 2B, RABL2B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RABL2B Polyclonal antibody (11588-1-AP)

    WB analysis using 11588-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11588-1-AP (RABL2B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using RABL2B Polyclonal antibody (11588-1-AP)

    WB analysis of human brain using 11588-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11588-1-AP (RABL2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using RABL2B Polyclonal antibody (11588-1-AP)

    WB analysis of human kidney using 11588-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 11588-1-AP (RABL2B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using RABL2B Polyclonal antibody (11588-1-AP)

    WB analysis of human liver using 11588-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 11588-1-AP (RABL2B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using RABL2B Polyclonal antibody (11588-1-AP)

    WB analysis of human heart using 11588-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 11588-1-AP (RABL2B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of fetal human brain tissue using RABL2B Polyclonal antibody (11588-1-AP)

    IP experiment of fetal human brain using 11588-1-AP

    IP result of anti-RABL2B (IP:11588-1-AP, 4ug; Detection:11588-1-AP 1:300) with fetal human brain tissue lysate 4000 ug.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using RABL2B Polyclonal antibody (11588-1-AP)

    IHC staining of mouse kidney using 11588-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11588-1-AP (RABL2B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using RABL2B Polyclonal antibody (11588-1-AP)

    IHC staining of mouse kidney using 11588-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11588-1-AP (RABL2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RABL2B Polyclonal antibody (11588-1-AP)

    IHC staining of mouse brain using 11588-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11588-1-AP (RABL2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RABL2B Polyclonal antibody (11588-1-AP)

    IHC staining of human lung cancer using 11588-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 11588-1-AP (RABL2B antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected infetal human brain tissue, human brain tissue, human kidney tissue, human liver tissue, human heart tissue, mouse brain tissue
Positive IP detected infetal human brain tissue
Positive IHC detected inmouse kidney tissue, human lung cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11588-1-AP targets RABL2B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RABL2B fusion protein Ag2189
Full Name RAB, member of RAS oncogene family-like 2B
Calculated Molecular Weight 229 aa, 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC024281
Gene Symbol RABL2B
Gene ID (NCBI) 11158
RRIDAB_2175954
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RABL2B antibody 11588-1-APDownload protocol
IHC protocol for RABL2B antibody 11588-1-APDownload protocol
IP protocol for RABL2B antibody 11588-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

J Cell Sci

RABL2 positively controls localization of GPCRs in mammalian primary cilia.

Authors - Izumi Dateyama
View Article
humanWB

bioRxiv

Role of U11/U12 minor spliceosome gene ZCRB1 in Ciliogenesis and WNT Signaling

Authors - Geralle Powell-Rodgers
View Article