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Tested Applications
| Positive WB detected in | mouse pancreas tissue, human brain tissue, mouse brain tissue |
| Positive IHC detected in | mouse ovary tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 2 publications below |
| IP | See 1 publications below |
| RIP | See 1 publications below |
Product Information
17816-1-AP targets RABL2A in WB, IP, RIP, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RABL2A fusion protein Ag12135 Predict reactive species |
| Full Name | RAB, member of RAS oncogene family-like 2A |
| Calculated Molecular Weight | 165aa,18 kDa; 228aa,26 kDa |
| Observed Molecular Weight | 26 kDa |
| GenBank Accession Number | BC111008 |
| Gene Symbol | RABL2A |
| Gene ID (NCBI) | 11159 |
| RRID | AB_2175953 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UBK7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RABL2A antibody 17816-1-AP | Download protocol |
| IHC protocol for RABL2A antibody 17816-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
DNA Cell Biol RABL2A-CCDC34 Axis Promotes Sorafenib Resistance in Hepatocellular Carcinoma.
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