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Peripherin Monoclonal antibody

Peripherin Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat, pig

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1H10B3

Cat no : 66317-1-Ig

Synonyms

PRPH, NEF4, 1H10B3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Peripherin Monoclonal antibody (66317-1-Ig)

    WB analysis using 66317-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using Peripherin Monoclonal antibody (66317-1-Ig)

    WB analysis of rat brain using 66317-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human colon tissue using Peripherin Monoclonal antibody (66317-1-Ig)

    WB analysis of human colon using 66317-1-Ig

    human colon tissue were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat colon tissue using Peripherin Monoclonal antibody (66317-1-Ig)

    WB analysis of rat colon using 66317-1-Ig

    rat colon tissue were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using Peripherin Monoclonal antibody (66317-1-Ig)

    IHC staining of human brain using 66317-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66317-1-Ig (Peripherin antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using Peripherin Monoclonal antibody (66317-1-Ig)

    IHC staining of human brain using 66317-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66317-1-Ig (Peripherin antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using Peripherin Monoclonal antibody (66317-1-Ig)

    IHC staining of human colon using 66317-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66317-1-Ig (Peripherin Antibody) at dilution of 1:1000 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using Peripherin Monoclonal antibody (66317-1-Ig)

    IHC staining of human colon using 66317-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66317-1-Ig (Peripherin Antibody) at dilution of 1:1000 (under 10x lens).

  • Flow cytometry (FC) experiment of PC-12 cells using Peripherin Monoclonal antibody (66317-1-Ig)

    FC experiment of PC-12 using 66317-1-Ig

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human Peripherin (66317-1-Ig, Clone:1H10B3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inIMR-32 cells, human colon tissue, rat brain tissue, rat colon tissue, mouse colon tissue, rat spinal cord tissue
Positive IHC detected inhuman brain tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inPC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66317-1-Ig targets Peripherin in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, rat, pig samples.

Tested Reactivity human, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peripherin fusion protein Ag11116
Full Name peripherin
Calculated Molecular Weight 470 aa, 54 kDa
Observed Molecular Weight 50-54 kDa
GenBank Accession NumberBC032703
Gene Symbol Peripherin
Gene ID (NCBI) 5630
RRIDAB_2881698
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Peripherin antibody 66317-1-IgDownload protocol
IHC protocol for Peripherin antibody 66317-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols