Filter: AllWBIHCFC (Intra)

WB analysis using 66317-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 66317-1-Ig
rat brain tissue were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of human colon using 66317-1-Ig
human colon tissue were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of rat colon using 66317-1-Ig
rat colon tissue were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of human brain using 66317-1-Ig
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66317-1-Ig (Peripherin antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human brain using 66317-1-Ig
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66317-1-Ig (Peripherin antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human colon using 66317-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66317-1-Ig (Peripherin Antibody) at dilution of 1:1000 (under 40x lens).
IHC staining of human colon using 66317-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66317-1-Ig (Peripherin Antibody) at dilution of 1:1000 (under 10x lens).
FC experiment of PC-12 using 66317-1-Ig
1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human Peripherin (66317-1-Ig, Clone:1H10B3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Peripherin Monoclonal antibody

Name: Peripherin Monoclonal antibody
Brand: Proteintech
SKU: 66317-1-Ig

Peripherin Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 66317-1-Ig

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat, pig

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

PRPH, NEF4, 1H10B3

Formulation: PBS and Azide

PBS and Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	IMR-32 cells, human colon tissue, rat brain tissue, rat colon tissue, mouse colon tissue, rat spinal cord tissue
Positive IHC detected in	human brain tissue, human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC (Intra) detected in	PC-12 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66317-1-Ig targets Peripherin in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, rat, pig samples.

Tested Reactivity	human, rat, pig
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	Peripherin fusion protein Ag11116 Predict reactive species
Full Name	peripherin
Calculated Molecular Weight	470 aa, 54 kDa
Observed Molecular Weight	50-54 kDa
GenBank Accession Number	BC032703
Gene Symbol	Peripherin
Gene ID (NCBI)	5630
RRID	AB_2881698
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P41219
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.