Paxillin Polyclonal antibody
Paxillin Polyclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, Canine, mouse
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 22172-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | RAW 264.7 cells |
| Positive IHC detected in | human breast cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MDCK cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
| IHC | See 1 publications below |
Product Information
The immunogen of 22172-1-AP is Paxillin Fusion Protein expressed in E. coli.
| Tested Reactivity | human, Canine, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Paxillin fusion protein Ag17702 |
| Full Name | paxillin |
| Calculated Molecular Weight | 591 aa, 65 kDa |
| GenBank Accession Number | BC136787 |
| Gene Symbol | Paxillin |
| Gene ID (NCBI) | 5829 |
| RRID | AB_2879016 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Paxillin antibody 22172-1-AP | Download protocol |
| IHC protocol for Paxillin antibody 22172-1-AP | Download protocol |
| IF protocol for Paxillin antibody 22172-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncogene KIF26B, a novel oncogene, promotes proliferation and metastasis by activating the VEGF pathway in gastric cancer. | ||
Sci Rep A novel cystathionine γ-lyase inhibitor, I194496, inhibits the growth and metastasis of human TNBC via downregulating multiple signaling pathways. | ||
Sci Rep Interferon-inducible protein, IFIX, has tumor-suppressive effects in oral squamous cell carcinoma | ||
Int J Oncol Cystathionine‑γ‑lyase promotes the metastasis of breast cancer via the VEGF signaling pathway. | ||
Cell Death Discov A novel chemical inhibitor suppresses breast cancer cell growth and metastasis through inhibiting HPIP oncoprotein. | ||
Cell Signal Activated CRH receptors inhibit autophagy by repressing conversion of LC3BI to LC3BII. |
