PTPRR Polyclonal antibody
PTPRR Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 17937-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, mouse brain tissue, COLO 320 cells, SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
17937-1-AP targets PTPRR in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PTPRR fusion protein Ag12145 |
| Full Name | protein tyrosine phosphatase, receptor type, R |
| Calculated Molecular Weight | 657 aa, 74 kDa |
| Observed Molecular Weight | 32 kDa, 47 kDa, 65 kDa |
| GenBank Accession Number | BC110900 |
| Gene Symbol | PTPRR |
| Gene ID (NCBI) | 5801 |
| RRID | AB_10694430 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PTPRR(protein tyrosine phosphatase, receptor type, R) is also named as PCPTP1, PTP-SL, PTPBR7 and belongs to the protein-tyrosine phosphatase family. It sequesters mitogen-activated protein kinases (MAPKs) such as MAPK1, MAPK3 and MAPK14 in the cytoplasm in an inactive form. It has four isoforms that they have molecular weights of 72, 60, 42 and 37 kDa, respectively, and differ in the length of their N-terminal part which result in distinct subcellular localizations. All PTPRR isoforms are found to be short-lived proteins, with half-lives of around 3-5 hours, and are constitutively phosphorylated on one or two of the previously identified protein kinase A and MAP kinase target sites.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PTPRR antibody 17937-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cancer Multi-cohort validation of Ascore: an anoikis-based prognostic signature for predicting disease progression and immunotherapy response in bladder cancer | ||
BMC Cancer Androgen-regulation of the protein tyrosine phosphatase PTPRR activates ERK1/2 signalling in prostate cancer cells. | ||
Front Mol Neurosci Downregulation of Protein Tyrosine Phosphatase Receptor Type R Accounts for the Progression of Hirschsprung Disease. |
