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PTPN5 Polyclonal antibody

PTPN5 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14515-1-AP

Synonyms

PTPN5, PTPSTEP, STEP

Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using PTPN5 Polyclonal antibody (14515-1-AP)

    WB analysis of HL-60 using 14515-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 14515-1-AP (PTPN5 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using PTPN5 Polyclonal antibody (14515-1-AP)

    IP experiment of mouse brain using 14515-1-AP

    IP result of anti-PTPN5 (IP:14515-1-AP, 4ug; Detection:14515-1-AP 1:1500) with mouse brain tissue lysate 1840 ug.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using PTPN5 Polyclonal antibody (14515-1-AP)

    IHC staining of mouse spleen using 14515-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 14515-1-AP (PTPN5 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using PTPN5 Polyclonal antibody (14515-1-AP)

    IHC staining of mouse spleen using 14515-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 14515-1-AP (PTPN5 Antibody) at dilution of 1:200 (under 10x lens).

Tested Applications

Positive WB detected inHL-60 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14515-1-AP targets PTPN5 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTPN5 fusion protein Ag5974
Full Name protein tyrosine phosphatase, non-receptor type 5 (striatum-enriched)
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 46-50 kDa, 61-66 kDa
GenBank Accession NumberBC064807
Gene Symbol PTPN5
Gene ID (NCBI) 84867
RRIDAB_2173548
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PTPN5 antibody 14515-1-APDownload protocol
IHC protocol for PTPN5 antibody 14515-1-APDownload protocol
IP protocol for PTPN5 antibody 14515-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Mol Neurobiol

The Weakened Interaction Between HECTD4 and GluN2B in Ischemic Stroke Promotes Calcium Overload and Brain Injury Through a Mechanism Involving the Decrease of GluN2B and MALT1 Ubiquitination

Authors - Yi-Yue Zhang
View Article