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PRL3 Polyclonal antibody

PRL3 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15186-1-AP

Synonyms

PTP4A3, Protein-tyrosine phosphatase 4a3, PRL-R, PRL-3, PRL R

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using PRL3 Polyclonal antibody (15186-1-AP)

    WB analysis using 15186-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15186-1-AP (PRL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PRL3 Polyclonal antibody (15186-1-AP)

    IHC staining of human colon cancer using 15186-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15186-1-AP (PRL3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PRL3 Polyclonal antibody (15186-1-AP)

    IHC staining of human colon cancer using 15186-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15186-1-AP (PRL3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PRL3 Polyclonal antibody (15186-1-AP)

    IHC staining of human colon cancer using 15186-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15186-1-AP (PRL3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PRL3 Polyclonal antibody (15186-1-AP)

    IHC staining of human colon cancer using 15186-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15186-1-AP (PRL3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using PRL3 Polyclonal antibody (15186-1-AP)

    IHC staining of human oesophagus cancer using 15186-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15186-1-AP (PRL3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PRL3 Polyclonal antibody (15186-1-AP)

    IHC staining of human colon cancer using 15186-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15186-1-AP (PRL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PRL3 Polyclonal antibody (15186-1-AP)

    IHC staining of human colon cancer using 15186-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15186-1-AP (PRL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using PRL3 Polyclonal antibody (15186-1-AP)

    IHC staining of human oesophagus cancer using 15186-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer using 15186-1-AP (PTP4A3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using PRL3 Polyclonal antibody (15186-1-AP)

    IHC staining of human oesophagus cancer using 15186-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer using 15186-1-AP (PTP4A3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using PRL3 Polyclonal antibody (15186-1-AP)

    IHC staining of human heart using 15186-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 15186-1-AP (PTP4A3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using PRL3 Polyclonal antibody (15186-1-AP)

    IHC staining of human heart using 15186-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 15186-1-AP (PTP4A3 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse heart tissue, rat heart tissue
Positive IHC detected inhuman colon cancer tissue, human oesophagus cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15186-1-AP targets PRL3 in IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRL3 fusion protein Ag7249
Full Name protein tyrosine phosphatase type IVA, member 3
Calculated Molecular Weight 20 kDa
Observed Molecular Weight 20-28 kDa
GenBank Accession NumberBC003105
Gene Symbol PTP4A3
Gene ID (NCBI) 11156
RRIDAB_2878115
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phosphatase of regenerating liver-3 (PRL-3; also known as PTP4A3), encodes a 22-kDa protein that represents a novel class of a superfamily of protein tyrosine phosphatases (PTPs) that are ubiquitously expressed in various tumors. PRL3 was associated with primary tumor growth, tumor reoccurrence, and therapy resistance in many human cancers. This protein was also suggested as a marker to predict the relapse of gastric cancer and invasive breast cancer. (PMID: 22469786, PMID: 30157875)

Protocols

Product Specific Protocols
WB protocol for PRL3 antibody 15186-1-APDownload protocol
IHC protocol for PRL3 antibody 15186-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Reprod Sci

PRL-3 Is Involved in Estrogen- and IL-6-Induced Migration of Endometrial Stromal Cells From Ectopic Endometrium.

Authors - Shifan Ren
View Article
humanIF

Mol Cancer

Super-enhancer-driven TOX2 mediates oncogenesis in Natural Killer/T Cell Lymphoma

Authors - Jianbiao Zhou
View Article