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PTCD3 Polyclonal antibody

PTCD3 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 25158-1-AP

Synonyms

PTCD3, TRG 15

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse embryo tissue using PTCD3 Polyclonal antibody (25158-1-AP)

    WB analysis of mouse embryo using 25158-1-AP

    mouse embryo tissue were subjected to SDS PAGE followed by western blot with 25158-1-AP (PTCD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat heart tissue using PTCD3 Polyclonal antibody (25158-1-AP)

    WB analysis of rat heart using 25158-1-AP

    rat heart tissue were subjected to SDS PAGE followed by western blot with 25158-1-AP (PTCD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat kidney tissue using PTCD3 Polyclonal antibody (25158-1-AP)

    WB analysis of rat kidney using 25158-1-AP

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 25158-1-AP (PTCD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PTCD3 Polyclonal antibody (25158-1-AP)

    WB analysis of HEK-293 using 25158-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25158-1-AP (PTCD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using PTCD3 Polyclonal antibody (25158-1-AP)

    IP experiment of HEK-293 using 25158-1-AP

    IP result of anti-PTCD3 (IP:25158-1-AP, 4ug; Detection:25158-1-AP 1:600) with HEK-293 cells lysate 3600ug.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using PTCD3 Polyclonal antibody (25158-1-AP)

    IF Staining of HEK-293 using 25158-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 25158-1-AP (PTCD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse embryo tissue, HEK-293 cells, rat heart tissue, rat kidney tissue
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

The immunogen of 25158-1-AP is PTCD3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTCD3 fusion protein Ag18330
Full Name Pentatricopeptide repeat domain 3
Calculated Molecular Weight 689 aa, 79 kDa
Observed Molecular Weight 78-80 kDa
GenBank Accession NumberBC011832
Gene Symbol PTCD3
Gene ID (NCBI) 55037
RRIDAB_2879931
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PTCD3 antibody 25158-1-APDownload protocol
IF protocol for PTCD3 antibody 25158-1-APDownload protocol
IP protocol for PTCD3 antibody 25158-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

MRPS31 loss is a key driver of mitochondrial deregulation and hepatocellular carcinoma aggressiveness.

Authors - Seongki Min
View Article
humanWB

Biomedicines

Mitochondrial Ribosome Dysfunction in Human Alveolar Type II Cells in Emphysema

Authors - Loukmane Karim
View Article
humanWB

J Gen Virol

Inhibition of human cytomegalovirus replication by interferon alpha can involve multiple anti-viral factors

Authors - Shabab Chowdhury
View Article