PSTPIP1 Polyclonal antibody
PSTPIP1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 11951-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | THP-1 cells, mouse thymus tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
Product Information
11951-1-AP targets PSTPIP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PSTPIP1 fusion protein Ag2551 |
| Full Name | proline-serine-threonine phosphatase interacting protein 1 |
| Calculated Molecular Weight | 416 aa, 48 kDa |
| Observed Molecular Weight | 45-48 kDa |
| GenBank Accession Number | BC008602 |
| Gene Symbol | PSTPIP1 |
| Gene ID (NCBI) | 9051 |
| RRID | AB_10694665 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PSTPIP1 (proline-serine-threonine phosphatase interacting protein 1), also known as CD2BP1, is a cytoskeleton-associated adaptor protein that links PEST-type phosphatases to their substrates (PMID: 19584923). It is involved in regulation of the actin cytoskeleton. Preferentially expressed in hematopoetic tissues, PSTPIP1 binds to the cytoplasmic tail of CD2, negatively affecting CD2-triggered T cell activation (PMID: 16670297). It has also been shown to interact with ABL1, PTPN18, WAS, CD2AP, PTPN12 and MEFV/pyrin. Mutations in the gene of PSTPIP1 cause PAPA syndrome (Pyogenic sterile Arthritis, Pyoderma gangrenosum, and Acne), an autoinflammatory disease (PMID:11971877; 14595024).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PSTPIP1 antibody 11951-1-AP | Download protocol |
| IHC protocol for PSTPIP1 antibody 11951-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
AMB Express Quantitative proteomics revealed modulation of macrophages by MetQ gene of Streptococcus suis serotype 2. | ||
Front Genet Identification of Novel Prognostic Biomarkers Relevant to Immune Infiltration in Lung Adenocarcinoma. |
