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PSMG2 Polyclonal antibody

PSMG2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 10959-1-AP

Synonyms

PAC2, MDS003, HsT1707, Hepatocellular carcinoma-susceptibility protein 3, HCCA3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMG2 Polyclonal antibody (10959-1-AP)

    WB analysis using 10959-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10959-1-AP (PSMG2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10959-1-AP targets PSMG2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSMG2 fusion protein Ag1400
Full Name proteasome (prosome, macropain) assembly chaperone 2
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC013356
Gene Symbol PSMG2
Gene ID (NCBI) 56984
RRIDAB_2171454
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Proteasome assembly chaperone 2 (PSMG2), also named as Hepatocellular carcinoma-susceptibility protein 3, Tumor necrosis factor superfamily member 5-induced protein 1. It has a function as a chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG1. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha subunits into the heteroheptameric alpha ring and prevents alpha ring dimerization.It is widely expressed with highest levels in lung, brain and colon,moderately expressed in muscle, stomach, spleen and heart and weakly expressed in small intestine, pancreas and liver,highly expressed in hepatocellular carcinomas with low levels in surrounding liver tissue. Molecular mass of PSMG2 is 29kD.

Protocols

Product Specific Protocols
WB protocol for PSMG2 antibody 10959-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols