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PSAP Monoclonal antibody
PSAP Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
2F11B2
Cat no : 66473-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human milk tissue, human milk, HepG2 cells, PC-3 cells, HeLa cells |
Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human prostate cancer tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2500-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
66473-1-Ig targets PSAP in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | PSAP fusion protein Ag1194 |
Full Name | prosaposin |
Calculated Molecular Weight | 58 kDa |
Observed Molecular Weight | 70 kDa |
GenBank Accession Number | BC001503 |
Gene Symbol | PSAP |
Gene ID (NCBI) | 5660 |
RRID | AB_2881839 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for PSAP antibody 66473-1-Ig | Download protocol |
IHC protocol for PSAP antibody 66473-1-Ig | Download protocol |
IF protocol for PSAP antibody 66473-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |