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PSAP Monoclonal antibody

PSAP Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2F11B2

Cat no : 66473-1-Ig

Synonyms

CSAct, Component C, Co beta glucosidase, Cerebroside sulfate activator, A1 activator

Validation Data Gallery

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  • Western Blot (WB) analysis of human milk using PSAP Monoclonal antibody (66473-1-Ig)

    WB analysis of human milk using 66473-1-Ig

    human milk were subjected to SDS PAGE followed by western blot with 66473-1-Ig (PSAP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PSAP Monoclonal antibody (66473-1-Ig)

    WB analysis of HeLa using 66473-1-Ig

    WB result of PSAP antibody (66473-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSAP transfected HeLa cells.

  • Western Blot (WB) analysis of HepG2 cells using PSAP Monoclonal antibody (66473-1-Ig)

    WB analysis of HepG2 using 66473-1-Ig

    WB result of PSAP antibody (66473-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSAP transfected HepG2 cells.

  • Western Blot (WB) analysis of HepG2 cells using PSAP Monoclonal antibody (66473-1-Ig)

    WB analysis of HepG2 using 66473-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66473-1-Ig (PSAP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using PSAP Monoclonal antibody (66473-1-Ig)

    WB analysis of PC-3 using 66473-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 66473-1-Ig (PSAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PSAP Monoclonal antibody (66473-1-Ig)

    IHC staining of human prostate cancer using 66473-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66473-1-Ig (PSAP antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PSAP Monoclonal antibody (66473-1-Ig)

    IHC staining of human prostate cancer using 66473-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66473-1-Ig (PSAP antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human prostate cancer tissue using PSAP Monoclonal antibody (66473-1-Ig)

    IF Staining of human prostate cancer using 66473-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using PSAP antibody (66473-1-Ig, Clone: 2F11B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human prostate cancer tissue using PSAP Monoclonal antibody (66473-1-Ig)

    IF Staining of human prostate cancer using 66473-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using PSAP antibody (66473-1-Ig, Clone: 2F11B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PSAP Monoclonal antibody (66473-1-Ig)

    IF Staining of HeLa using 66473-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSAP antibody (66473-1-Ig, Clone: 2F11B2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red).

Tested Applications

Positive WB detected inhuman milk tissue, human milk, HepG2 cells, PC-3 cells, HeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman prostate cancer tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2500-1:10000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66473-1-Ig targets PSAP in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PSAP fusion protein Ag1194
Full Name prosaposin
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC001503
Gene Symbol PSAP
Gene ID (NCBI) 5660
RRIDAB_2881839
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PSAP antibody 66473-1-IgDownload protocol
IHC protocol for PSAP antibody 66473-1-IgDownload protocol
IF protocol for PSAP antibody 66473-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols