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PSAP Polyclonal antibody

PSAP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 18398-1-AP

Synonyms

Dispersin, CSAct, Component C, Co beta glucosidase, Cerebroside sulfate activator

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PSAP Polyclonal antibody (18398-1-AP)

    WB analysis of HeLa using 18398-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18398-1-AP (PSAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PSAP Polyclonal antibody (18398-1-AP)

    WB analysis of HeLa using 18398-1-AP

    WB result of PSAP antibody (18398-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSAP transfected HeLa cells.

  • Western Blot (WB) analysis of HepG2 cells using PSAP Polyclonal antibody (18398-1-AP)

    WB analysis of HepG2 using 18398-1-AP

    WB result of PSAP antibody (18398-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSAP transfected HepG2 cells.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSAP Polyclonal antibody (18398-1-AP)

    IHC staining of human liver cancer using 18398-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 18398-1-AP (PSAP antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSAP Polyclonal antibody (18398-1-AP)

    IHC staining of human liver cancer using 18398-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 18398-1-AP (PSAP antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using PSAP Polyclonal antibody (18398-1-AP)

    IF Staining of PC-3 using 18398-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 18398-1-AP (PSAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18398-1-AP targets PSAP in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSAP fusion protein Ag13265
Full Name prosaposin
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC001503
Gene Symbol PSAP
Gene ID (NCBI) 5660
RRIDAB_10598315
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

proteins' (SAPs) that assist in the lysosomal hydrolysis of sphingolipids. After proteolytic processing of the presaposin protein, these 4 released polypeptides are functional activators. Saposin A was encoded by residues 60 to 143 of PSAP, saposin B by 195 to 275, saposin C by 311 to 390, and saposin D by 405 to 487. There are four 12-14 kDa heatstable glycoproteins. This antibody should recognize saposin C.

Protocols

Product Specific Protocols
WB protocol for PSAP antibody 18398-1-APDownload protocol
IHC protocol for PSAP antibody 18398-1-APDownload protocol
IF protocol for PSAP antibody 18398-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,CoIP

Hum Mol Genet

Lysosomal ceramides regulate Cathepsin B-mediated processing of saposin C and glucocerebrosidase activity.

Authors - Myung Jong Kim
View Article
humanWB

JCI Insight

Inhibition of cysteine protease cathepsin Lincreases the level and activity of lysosomal glucocerebrosidase

Authors - Myung Jong Kim
View Article