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PSAP Polyclonal antibody

PSAP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18396-1-AP

Synonyms

Dispersin, CSAct, Component C, Co beta glucosidase, Cerebroside sulfate activator

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using PSAP Polyclonal antibody (18396-1-AP)

    WB analysis of HepG2 using 18396-1-AP

    WB result of PSAP antibody (18396-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSAP transfected HepG2 cells.

  • Western Blot (WB) analysis of human liver tissue using PSAP Polyclonal antibody (18396-1-AP)

    WB analysis of human liver using 18396-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 18396-1-AP (PSAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PSAP Polyclonal antibody (18396-1-AP)

    WB analysis of HepG2 using 18396-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18396-1-AP (PSAP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSAP Polyclonal antibody (18396-1-AP)

    IHC staining of human liver cancer using 18396-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 18396-1-AP (PSAP antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSAP Polyclonal antibody (18396-1-AP)

    IHC staining of human liver cancer using 18396-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 18396-1-AP (PSAP antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PSAP Polyclonal antibody (18396-1-AP)

    IF Staining of HepG2 using 18396-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 18396-1-AP (PSAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, human liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18396-1-AP targets PSAP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSAP fusion protein Ag13263
Full Name prosaposin
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 65 kDa, 70 kDa
GenBank Accession NumberBC001503
Gene Symbol PSAP
Gene ID (NCBI) 5660
RRIDAB_2172460
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The PSAP gene encodes prosaposin, a precursor of fourl small nonenzymatic glycoproteins termed 'sphingolipid activator proteins' (SAPs) that assist in the lysosomal hydrolysis of sphingolipids. After proteolytic processing of the presaposin protein, these 4 released polypeptides are functional activators. Saposin A was encoded by residues 60 to 143 of PSAP, saposin B by 195 to 275, saposin C by 311 to 390, and saposin D by 405 to 487. There are four 12-14 kDa heatstable glycoproteins. This antibody should recognize saposin A.

Protocols

Product Specific Protocols
WB protocol for PSAP antibody 18396-1-APDownload protocol
IHC protocol for PSAP antibody 18396-1-APDownload protocol
IF protocol for PSAP antibody 18396-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols