Various lysates were subjected to SDS PAGE followed by western blot with 17399-1-AP (Peripherin antibody) at dilution of 1:120000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 17399-1-AP (Peripherin antibody) at dilution of 1:120000 incubated at room temperature for 1.5 hours.
WB analysis of mouse colon using 17399-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 17399-1-AP (PRPH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
mouse colon tissue were subjected to SDS PAGE followed by western blot with 17399-1-AP (PRPH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 17399-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 17399-1-AP (Peripherin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
COLO 320 cells were subjected to SDS PAGE followed by western blot with 17399-1-AP (Peripherin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human colon using 17399-1-AP
human colon tissue were subjected to SDS PAGE followed by western blot with 17399-1-AP (PRPH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
human colon tissue were subjected to SDS PAGE followed by western blot with 17399-1-AP (PRPH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of mouse brain using 17399-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17399-1-AP (Peripherin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17399-1-AP (Peripherin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 17399-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17399-1-AP (Peripherin Antibody) at dilution of 1:50 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17399-1-AP (Peripherin Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon using 17399-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17399-1-AP (Peripherin Antibody) at dilution of 1:50 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17399-1-AP (Peripherin Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of rat brain using 17399-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 17399-1-AP (Peripherin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 17399-1-AP (Peripherin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 17399-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 17399-1-AP (PRPH antibody) at dilution of 1:100 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human brain using 17399-1-AP (PRPH antibody) at dilution of 1:100 (under 40x lens).
IF Staining of human colon using 17399-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using Peripherin antibody (17399-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using Peripherin antibody (17399-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human colon using 17399-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using Peripherin antibody (17399-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using Peripherin antibody (17399-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC experiment of PC-12 using 17399-1-AP
1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human Peripherin (17399-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human Peripherin (17399-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
mouse colon tissue, COLO 320 cells, human colon tissue, rat colon tissue, rat spinal cord tissue
Positive IHC detected in
mouse brain tissue, human colon tissue, human brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected in
human colon tissue
Positive FC (Intra) detected in
PC-12 cells
Positive FC detected in
PC-12 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:20000-1:100000
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)-P
IF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)
FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Peripherin is a neuron-specific intermediate filament protein that is particularly expressed in peripheral nervous system. It is used to study peripheral nerve development and regeneration, as it is highly expressed during elongation of axon. Peripherin is a routine marker for unmyelinated neurons in trigeminal ganglion (TG) and the dorsal root ganglion (DRG). Peripherin is also expressed in peri-insular areas of islets and it has been identified as a key autoantigen for type 1A diabetes.
Various lysates were subjected to SDS PAGE followed by western blot with 17399-1-AP (Peripherin antibody) at dilution of 1:120000 incubated at room temperature for 1.5 hours.
WB analysis of mouse colon using 17399-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 17399-1-AP (PRPH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 17399-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 17399-1-AP (Peripherin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human colon using 17399-1-AP
human colon tissue were subjected to SDS PAGE followed by western blot with 17399-1-AP (PRPH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse brain using 17399-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17399-1-AP (Peripherin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 17399-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17399-1-AP (Peripherin Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon using 17399-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17399-1-AP (Peripherin Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of rat brain using 17399-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 17399-1-AP (Peripherin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 17399-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 17399-1-AP (PRPH antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human brain using 17399-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 17399-1-AP (PRPH antibody) at dilution of 1:100 (under 40x lens).
IF-P Figures
IF Staining of human colon using 17399-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using Peripherin antibody (17399-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human colon using 17399-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using Peripherin antibody (17399-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC (INTRA) Figures
FC experiment of PC-12 using 17399-1-AP
1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human Peripherin (17399-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
Peripherin Polyclonal antibody
Catalog Number
17399-1-AP
Citations
1
Dilutions
WB : 1:20000-1:100000 IHC : 1:50-1:500 IF-P : 1:50-1:500 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF-P, FC (Intra), ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
