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PROSC Polyclonal antibody

PROSC Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25154-1-AP

Synonyms

PLPBP, Pyridoxal phosphate-binding protein, Pyridoxal phosphate homeostasis protein, Proline synthase co-transcribed bacterial homolog protein, PLP homeostasis protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PROSC Polyclonal antibody (25154-1-AP)

    WB analysis using 25154-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25154-1-AP (PROSC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PROSC Polyclonal antibody (25154-1-AP)

    WB analysis of HepG2 using 25154-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25154-1-AP (PROSC antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using PROSC Polyclonal antibody (25154-1-AP)

    WB analysis of L02 using 25154-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 25154-1-AP (PROSC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using PROSC Polyclonal antibody (25154-1-AP)

    IHC staining of human skeletal muscle using 25154-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25154-1-AP (PROSC Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using PROSC Polyclonal antibody (25154-1-AP)

    IHC staining of human skeletal muscle using 25154-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25154-1-AP (PROSC Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using PROSC Polyclonal antibody (25154-1-AP)

    IF Staining of L02 using 25154-1-AP

    Immunofluorescent analysis of L02 cells using 25154-1-AP (PROSC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, L02 cells
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inL02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

25154-1-AP targets PROSC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PROSC fusion protein Ag18369
Full Name proline synthetase co-transcribed homolog (bacterial)
Calculated Molecular Weight 275 aa, 30 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC012334
Gene Symbol PROSC
Gene ID (NCBI) 11212
RRIDAB_2879929
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human Pyridoxal 5′-phosphate-binding protein (PLPBP; previously proline synthase cotranscribed homolog, PROSC) was first described in 1999 and was demonstrated to be ubiquitously expressed in many tissues. PROSC is ubiquitously expressed in human tissues and is highly conserved throughout evolution. PROSC deficiency could therefore markedly increase free cellular PLP levels (PMID: 31825581, 27912044).

Protocols

Product Specific Protocols
WB protocol for PROSC antibody 25154-1-APDownload protocol
IHC protocol for PROSC antibody 25154-1-APDownload protocol
IF protocol for PROSC antibody 25154-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Brain

PLPHP deficiency: clinical, genetic, biochemical, and mechanistic insights.

Authors - Devon L Johnstone
View Article
mouseWB

Int J Mol Sci

Translation Fidelity and Respiration Deficits in CLPP-Deficient Tissues: Mechanistic Insights from Mitochondrial Complexome Profiling

Authors - Jana Key
View Article