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PRM2 Polyclonal antibody

PRM2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA, IF

Conjugate

Unconjugated

Cat no : 14500-1-AP

Synonyms

Sperm histone HP2, Protamine-2, Basic nuclear protein HPS2, Basic nuclear protein HPS1, Basic nuclear protein HPI2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human testis tissue using PRM2 Polyclonal antibody (14500-1-AP)

    WB analysis of human testis using 14500-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 14500-1-AP (PRM2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using PRM2 Polyclonal antibody (14500-1-AP)

    IHC staining of human testis using 14500-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14500-1-AP (PRM2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using PRM2 Polyclonal antibody (14500-1-AP)

    IHC staining of human testis using 14500-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14500-1-AP (PRM2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14500-1-AP targets PRM2 in WB, IHC, ELISA, IF applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRM2 fusion protein Ag5952
Full Name protamine 2
Calculated Molecular Weight 13 kDa
Observed Molecular Weight 10-25 kDa
GenBank Accession NumberBC066338
Gene Symbol PRM2
Gene ID (NCBI) 5620
RRIDAB_2721024
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRM2 (Protamine-2) is the main DNA-binding protein in the sperm nucleus, consisting of a C-terminal mature PRM2 (mPRM2) domain and an N-terminal lytic PRM2 (cPRM2) domain, which is lyzed sequentially after binding to DNA. PRM2 plays an important role in spermatogenesis and development. PRM2 is specifically expressed in sperm and localized in the sperm nucleus.

Protocols

Product Specific Protocols
WB protocol for PRM2 antibody 14500-1-APDownload protocol
IHC protocol for PRM2 antibody 14500-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Chromodomain Protein CDYL Acts as a Crotonyl-CoA Hydratase to Regulate Histone Crotonylation and Spermatogenesis.

Authors - Shumeng Liu
View Article
mouseIF

J Hazard Mater

Multi-omics analyses reveal the mechanisms of Arsenic-induced male reproductive toxicity in mice.

Authors - Zijun Peng
View Article
mouseWB

Environ Pollut

Silica nanoparticles induce spermatogenesis disorders via L3MBTL2-DNA damage-p53 apoptosis and RNF8-ubH2A/ubH2B pathway in mice.

Authors - Jianhui Liu
View Article
mouseWB

Environ Pollut

Silica nanoparticles inhibiting the differentiation of round spermatid and chromatin remodeling of haploid period via MIWI in mice.

Authors - Jianhui Liu
View Article
humanWB

Hum Reprod

Lysine glutarylation in human sperm is associated with progressive motility.

Authors - Yi-Min Cheng
View Article
human

Hum Reprod

Posttranslational lysine 2-hydroxyisobutyrylation of human sperm tail proteins affects motility.

Authors - Yi-Min Cheng
View Article