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  • Featured Product
  • KD/KO Validated

PRKAR2A Polyclonal antibody

PRKAR2A Polyclonal Antibody for WB, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Cat no : 10142-2-AP

Synonyms

PRKAR2, PKR2, cAMP-dependent protein kinase type II-alpha regulatory subunit

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRKAR2A Polyclonal antibody (10142-2-AP)

    WB analysis using 10142-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10142-2-AP (PRKAR2A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PRKAR2A Polyclonal antibody (10142-2-AP)

    WB analysis of HeLa using 10142-2-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10142-2-AP (PRKAR2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using PRKAR2A Polyclonal antibody (10142-2-AP)

    WB analysis of K-562 using 10142-2-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 10142-2-AP (PRKAR2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using PRKAR2A Polyclonal antibody (10142-2-AP)

    WB analysis of mouse testis using 10142-2-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 10142-2-AP (PRKAR2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using PRKAR2A Polyclonal antibody (10142-2-AP)

    WB analysis of PC-3 using 10142-2-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 10142-2-AP (PRKAR2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using PRKAR2A Polyclonal antibody (10142-2-AP)

    WB analysis of human testis using 10142-2-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 10142-2-AP (PRKAR2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using PRKAR2A Polyclonal antibody (10142-2-AP)

    WB analysis of MCF-7 using 10142-2-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10142-2-AP (PRKAR2A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using PRKAR2A Polyclonal antibody (10142-2-AP)

    IP experiment of HeLa cells using 10142-2-AP

    IP result of anti-PRKAR2A (10142-2-AP for IP and Detection).

  • Immunoprecipitation (IP) experiment of HeLa cells using PRKAR2A Polyclonal antibody (10142-2-AP)

    IP experiment of HeLa using 10142-2-AP

    IP result of anti-PRKAR2A (IP:10142-2-AP, 4ug; Detection:10142-2-AP 1:2000) with HeLa cells lysate 1320 ug.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using PRKAR2A Polyclonal antibody (10142-2-AP)

    IF Staining of mouse testis using 10142-2-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PRKAR2A antibody (10142-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PRKAR2A Polyclonal antibody (10142-2-AP)

    IF Staining of HeLa using 10142-2-AP

    Immunofluorescent analysis of HeLa cells using 10142-2-AP (PRKAR2A antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PRKAR2A Polyclonal antibody (10142-2-AP)

    IF Staining of MCF-7 using 10142-2-AP

    Immunofluorescent analysis of MCF-7 cells using 10142-2-AP (PRKAR2A antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PRKAR2A Polyclonal antibody (10142-2-AP)

    IF Staining of HepG2 using 10142-2-AP

    Immunofluorescent analysis of HepG2 cells using 10142-2-AP (PRKAR2A antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, K-562 cells, MCF-7 cells, mouse testis tissue, PC-3 cells, human testis tissue
Positive IP detected inHeLa cells, HeLa cells
Positive IF-P detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10142-2-AP targets PRKAR2A in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRKAR2A fusion protein Ag0203
Full Name protein kinase, cAMP-dependent, regulatory, type II, alpha
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 46-52 kDa
GenBank Accession NumberBC002763
Gene Symbol PRKAR2A
Gene ID (NCBI) 5576
RRIDAB_2168236
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The effects of cAMP in most tissues and cell types are mainly modulated via protein kinase A, a heterotetrameric protein complex consisting of two regulatory and two catalytic subunits. The regulatory subunit of cAMP-dependent protein kinase (PRKAR2A) is one of the regulatory subunits and the gene is located on chromosome region 3p21.3-p21.2. The expression of PRKAR2A is tightly regulated during spermatogenesis, a significant increase in expression of this gene was also found in the human myometrium during pregnancy. This antibody got two bands about 45 kDa and 50-55 kDa in western blotting, and the 50-55kDa may be caused by phosphorylation.

Protocols

Product Specific Protocols
WB protocol for PRKAR2A antibody 10142-2-APDownload protocol
IF protocol for PRKAR2A antibody 10142-2-APDownload protocol
IP protocol for PRKAR2A antibody 10142-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Lett

Depletion of LAMP3 enhances PKA-mediated VASP phosphorylation to suppress invasion and metastasis in esophageal squamous cell carcinoma.

Authors - Furong Huang
  • KD Validated
View Article
ratWB,IHC

Life Sci

TMT-based proteomics analysis to screen potential biomarkers of acute-phase TBI in rats.

Authors - Haochen Wang
View Article
human,mouseWB,IP

Cell Rep

STK25 inhibits PKA signaling by phosphorylating PRKAR1A

Authors - Xiaokan Zhang
View Article
mouseWB

J Transl Med

Nicotine restores olfactory function by activation of prok2R/Akt/FoxO3a axis in Parkinson's disease

Authors - Qinglong Guo
View Article