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PRKAR1B Polyclonal antibody

PRKAR1B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17991-1-AP

Synonyms

PRKAR1, PRKAR1B

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using PRKAR1B Polyclonal antibody (17991-1-AP)

    WB analysis of mouse brain using 17991-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17991-1-AP (PRKAR1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PRKAR1B Polyclonal antibody (17991-1-AP)

    WB analysis of HeLa using 17991-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17991-1-AP (PRKAR1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Transfected HEK-293 cells using PRKAR1B Polyclonal antibody (17991-1-AP)

    WB analysis of Transfected HEK-293 using 17991-1-AP

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 17991-1-AP (PRKAR1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PRKAR1B Polyclonal antibody (17991-1-AP)

    IHC staining of human breast cancer using 17991-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17991-1-AP (PRKAR1B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PRKAR1B Polyclonal antibody (17991-1-AP)

    IHC staining of human breast cancer using 17991-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17991-1-AP (PRKAR1B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse brain tissue, Transfected HEK-293 cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 17991-1-AP is PRKAR1B Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRKAR1B fusion protein Ag12426
Full Name protein kinase, cAMP-dependent, regulatory, type I, beta
Calculated Molecular Weight 381 aa, 43 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC026734
Gene Symbol PRKAR1B
Gene ID (NCBI) 5575
RRIDAB_2878478
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PRKAR1B antibody 17991-1-APDownload protocol
IHC protocol for PRKAR1B antibody 17991-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols