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PRICKLE3 Polyclonal antibody

PRICKLE3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19098-1-AP

Synonyms

LIM domain only protein 6, LMO 6, LMO6, prickle homolog 3 (Drosophila), Prickle like protein 3, PRICKLE3, Triple LIM domain protein 6

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using PRICKLE3 Polyclonal antibody (19098-1-AP)

    WB analysis of Jurkat using 19098-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19098-1-AP (PRICKLE3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using PRICKLE3 Polyclonal antibody (19098-1-AP)

    WB analysis of Jurkat using 19098-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19098-1-AP (PRICKLE3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PRICKLE3 Polyclonal antibody (19098-1-AP)

    WB analysis of HeLa using 19098-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19098-1-AP (PRICKLE3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using PRICKLE3 Polyclonal antibody (19098-1-AP)

    IHC staining of human colon using 19098-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 19098-1-AP (PRICKLE3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using PRICKLE3 Polyclonal antibody (19098-1-AP)

    IHC staining of human colon using 19098-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 19098-1-AP (PRICKLE3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using PRICKLE3 Polyclonal antibody (19098-1-AP)

    IHC staining of human heart using 19098-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 19098-1-AP (PRICKLE3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using PRICKLE3 Polyclonal antibody (19098-1-AP)

    IHC staining of human heart using 19098-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 19098-1-AP (PRICKLE3 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inJurkat cells, HeLa cells
Positive IHC detected inhuman colon tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 19098-1-AP is PRICKLE3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRICKLE3 fusion protein Ag13632
Full Name prickle homolog 3 (Drosophila)
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC016856
Gene Symbol PRICKLE3
Gene ID (NCBI) 4007
RRIDAB_10644341
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PRICKLE3 antibody 19098-1-APDownload protocol
IHC protocol for PRICKLE3 antibody 19098-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols