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KD/KO Validated

PRDX6 Polyclonal antibody

Name: PRDX6 Polyclonal antibody
Brand: Proteintech
SKU: 13585-1-AP

PRDX6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13585-1-AP

Synonyms

EC:2.3.1.23, EC:1.11.1.27, AOP2, Acidic calcium-independent phospholipase A2, 1-Cys PRX

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCIF

WB analysis using 13585-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13585-1-AP (PRDX6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13585-1-AP
WB result of PRDX6 antibody (13585-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX6 transfected HeLa cells.
WB analysis of HAP1 using 13585-1-AP
HAP1 (WT and PRDX6 KO) lysates prepared with RIPA buffer, 25 μg protein loaded. 13585-1-AP incubated at 1:2000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.
WB analysis using 13585-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13585-1-AP (PRDX6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IP experiment of HAP1 using 13585-1-AP
HAP1 lysates prepared and IP of peroxidoxin 6 performed using 2.0 μg of 13585-1-AP coupled to protein A- Sepharose beads. Ponceau stained transfers shown for each blot. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.
IP experiment of HeLa using 13585-1-AP
IP result of anti-PRDX6 (IP:13585-1-AP, 3ug; Detection:13585-1-AP 1:500) with HeLa cells lysate 2800ug.
IHC staining of human brain using 13585-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 13585-1-AP (PRDX6 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 13585-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 13585-1-AP (PRDX6 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human colon using 13585-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13585-1-AP (PRDX6 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 13585-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13585-1-AP (PRDX6 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 13585-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 13585-1-AP (PRDX6 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 13585-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 13585-1-AP (PRDX6 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 13585-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed 48孔 HepG2 cells using PRDX6 antibody (13585-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 13585-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PRDX6 antibody (13585-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HAP1 using 13585-1-AP
HAP1 WT cells (yellow outline) and PRDX6 KO cells (red outline) labelled with a green or a far red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 13585-1-AP at 1:300 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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Cat No. 13585-1-AP

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Tested Applications

Positive WB detected in	SH-SY5Y cells, A549 cells, HeLa cells, HAP1, K-562 cells, HepG2 cells, mouse liver tissue, rat liver tissue
Positive IP detected in	HeLa cells, HAP1 cells
Positive IHC detected in	human brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	48孔 HepG2 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 28 publications below
IHC	See 7 publications below
IF	See 4 publications below

Product Information

13585-1-AP targets PRDX6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PRDX6 fusion protein Ag4512
Full Name	peroxiredoxin 6
Calculated Molecular Weight	224 aa, 25 kDa
Observed Molecular Weight	25-30 kDa
GenBank Accession Number	BC035857
Gene Symbol	PRDX6
Gene ID (NCBI)	9588
RRID	AB_2168637
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRDX6 (Peroxiredoxin-6), also named as AOP2 or KIAA0106, is a unique member of the peroxiredoxin family of antioxidants. PRDX6 is highly expressed in liver and protects cells from oxidative damage by reducing H2O2 and various lipid Peroxides (PMID: 17382207). It can form a dimer(PMID:20500660).PRDX6 is expressed in all major organs, with a particularly high level in lung (PMID:15890616). Prdx6 is detected at approximately 24 to 28 kDa, and can be monosumoylated with the molecular mass of about 40 kDa (PMID: 24910119).

Protocols

Product Specific Protocols
WB protocol for PRDX6 antibody 13585-1-AP	Download protocol
IHC protocol for PRDX6 antibody 13585-1-AP	Download protocol
IF protocol for PRDX6 antibody 13585-1-AP	Download protocol
IP protocol for PRDX6 antibody 13585-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun Suppression of mitochondrial ROS by prohibitin drives glioblastoma progression and therapeutic resistance. Authors - Haohao Huang View Article
human	WB	Nat Commun Functional screening implicates miR-371-3p and peroxiredoxin 6 in reversible tolerance to cancer drugs. Authors - Nisebita Sahu KO Validated View Article
rat	WB	Br J Pharmacol Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation. Authors - Bo Wei View Article
human	WB	Biomed Pharmacother Glycyrrhetinic acid inhibits non-small cell lung cancer via promotion of Prdx6- and caspase-3-mediated mitochondrial apoptosis Authors - Qiuyan Guo View Article
human	WB	Antioxid Redox Signal Peroxiredoxin 6 is a crucial factor in the initial step of mitochondrial clearance and is upstream of the PINK1-Parkin pathway. Authors - Shuaipeng Ma View Article
rat	WB,IHC	Environ Sci Pollut Res Int Curcumin strengthens a spontaneous self-protective mechanism-SP1/PRDX6 pathway, against di-n-butyl phthalate-induced testicular ferroptosis damage Authors - Hengtao Bu View Article

PRDX6 Polyclonal antibody

PRDX6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 13585-1-AP

WB analysis of HeLa using 13585-1-AP

WB analysis of HAP1 using 13585-1-AP

WB analysis using 13585-1-AP

IP experiment of HAP1 using 13585-1-AP

IP experiment of HeLa using 13585-1-AP

IHC staining of human brain using 13585-1-AP

IHC staining of human brain using 13585-1-AP

IHC staining of human colon using 13585-1-AP

IHC staining of human colon using 13585-1-AP

IHC staining of human brain using 13585-1-AP

IHC staining of human brain using 13585-1-AP

IF Staining of HepG2 using 13585-1-AP

IF Staining of HeLa using 13585-1-AP

IF Staining of HAP1 using 13585-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Suppression of mitochondrial ROS by prohibitin drives glioblastoma progression and therapeutic resistance.

Functional screening implicates miR-371-3p and peroxiredoxin 6 in reversible tolerance to cancer drugs.

Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation.

Glycyrrhetinic acid inhibits non-small cell lung cancer via promotion of Prdx6- and caspase-3-mediated mitochondrial apoptosis

Peroxiredoxin 6 is a crucial factor in the initial step of mitochondrial clearance and is upstream of the PINK1-Parkin pathway.

Curcumin strengthens a spontaneous self-protective mechanism-SP1/PRDX6 pathway, against di-n-butyl phthalate-induced testicular ferroptosis damage