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PPP4R2 Polyclonal antibody

PPP4R2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 22676-1-AP

Synonyms

PP4R2, PPP4R2

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using PPP4R2 Polyclonal antibody (22676-1-AP)

    WB analysis of A549 using 22676-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 22676-1-AP (PPP4R2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PPP4R2 Polyclonal antibody (22676-1-AP)

    WB analysis of HepG2 using 22676-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 22676-1-AP (PPP4R2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using PPP4R2 Polyclonal antibody (22676-1-AP)

    IP experiment of HepG2 using 22676-1-AP

    IP result of anti-PPP4R2 (IP:22676-1-AP, 4ug; Detection:22676-1-AP 1:1000) with HepG2 cells lysate 3600ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PPP4R2 Polyclonal antibody (22676-1-AP)

    IHC staining of human liver cancer using 22676-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22676-1-AP (PPP4R2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PPP4R2 Polyclonal antibody (22676-1-AP)

    IHC staining of human liver cancer using 22676-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22676-1-AP (PPP4R2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using PPP4R2 Polyclonal antibody (22676-1-AP)

    IHC staining of human pancreas using 22676-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22676-1-AP (PPP4R2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using PPP4R2 Polyclonal antibody (22676-1-AP)

    IHC staining of human pancreas using 22676-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22676-1-AP (PPP4R2 Antibody) at dilution of 1:50 (under 25x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PPP4R2 Polyclonal antibody (22676-1-AP)

    IF Staining of HepG2 using 22676-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 22676-1-AP (PPP4R2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22676-1-AP is PPP4R2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP4R2 fusion protein Ag18486
Full Name protein phosphatase 4, regulatory subunit 2
Calculated Molecular Weight360aa,40 kDa; 495aa,55 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC110889
Gene Symbol PPP4R2
Gene ID (NCBI) 151987
RRIDAB_2879149
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPP4R2 is a regulatory subunit of protein phosphatase-4 (PPP4), a protein serine/threonine phosphatase implicated in microtubule organization at centrosomes and DNA double-strand break repair. The deduced 453-amino acid protein contains several potential phosphorylation sites and has a calculated molecular mass of 50.4 kD. Immunofluorescent analysis of HeLa and A431 cells revealed prominent localization of PPP4R2 at centrosomes, with weaker staining of cytoplasm and nucleus(PMID: 10769191).

Protocols

Product Specific Protocols
WB protocol for PPP4R2 antibody 22676-1-APDownload protocol
IHC protocol for PPP4R2 antibody 22676-1-APDownload protocol
IF protocol for PPP4R2 antibody 22676-1-APDownload protocol
IP protocol for PPP4R2 antibody 22676-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,CoIP

Exp Cell Res

Protein phosphatase 4 mediates palmitic acid-induced endothelial dysfunction by decreasing eNOS phosphorylation at serine 633 in HUVECs

Authors - Zhengwei Liang
View Article