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PPP3R2 Polyclonal antibody

PPP3R2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14005-1-AP

Synonyms

Calcineurin B like protein, Calcineurin BII, Calcineurin subunit B type 2, CBLP, CNBII, PPP3R2, PPP3RL

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using PPP3R2 Polyclonal antibody (14005-1-AP)

    WB analysis of mouse testis using 14005-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14005-1-AP (PPP3R2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using PPP3R2 Polyclonal antibody (14005-1-AP)

    WB analysis of mouse testis using 14005-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14005-1-AP (PPP3R2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using PPP3R2 Polyclonal antibody (14005-1-AP)

    IP experiment of mouse testis using 14005-1-AP

    IP result of anti-PPP3R2 (IP:14005-1-AP, 4ug; Detection:14005-1-AP 1:500) with mouse testis tissue lysate 4000 ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using PPP3R2 Polyclonal antibody (14005-1-AP)

    IHC staining of human testis using 14005-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14005-1-AP (PPP3R2 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PPP3R2 Polyclonal antibody (14005-1-AP)

    IHC staining of human testis using 14005-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14005-1-AP (PPP3R2 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PPP3R2 Polyclonal antibody (14005-1-AP)

    IHC staining of human prostate cancer using 14005-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14005-1-AP (PPP3R2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PPP3R2 Polyclonal antibody (14005-1-AP)

    IHC staining of human prostate cancer using 14005-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14005-1-AP (PPP3R2 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman testis tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14005-1-AP targets PPP3R2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP3R2 fusion protein Ag5097
Full Name protein phosphatase 3 (formerly 2B), regulatory subunit B, beta isoform
Calculated Molecular Weight 20 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC030595
Gene Symbol PPP3R2
Gene ID (NCBI) 5535
RRIDAB_2168590
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PPP3R2 antibody 14005-1-APDownload protocol
IHC protocol for PPP3R2 antibody 14005-1-APDownload protocol
IP protocol for PPP3R2 antibody 14005-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Proc Natl Acad Sci U S A

SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice.

Authors - Haruhiko Miyata
View Article
mouseIHC

Cell Death Dis

miR-146a facilitates osteoarthritis by regulating cartilage homeostasis via targeting Camk2d and Ppp3r2.

Authors - Xudong Zhang
View Article
mouseIF, IHC, WB

FASEB J

Roles of glycogen synthase kinase 3 alpha and calcineurin in regulating the ability of sperm to fertilize eggs.

Authors - Souvik Dey
View Article
mouseWB

Eur J Pharmacol

Stachydrine hydrochloride suppresses phenylephrine-induced pathological cardiac hypertrophy by inhibiting the calcineurin/nuclear factor of activated T-cell signalling pathway.

Authors - Jiali Zheng
View Article