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PPP3CC Polyclonal antibody

PPP3CC Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19653-1-AP

Synonyms

CNA3, CAM-PRP catalytic subunit, CAM PRP catalytic subunit, CALNA3, Calmodulin-dependent calcineurin A subunit gamma isoform

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using PPP3CC Polyclonal antibody (19653-1-AP)

    WB analysis of mouse testis using 19653-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 19653-1-AP (PPP3CC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using PPP3CC Polyclonal antibody (19653-1-AP)

    WB analysis of mouse brain using 19653-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 19653-1-AP (PPP3CC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using PPP3CC Polyclonal antibody (19653-1-AP)

    WB analysis of MCF-7 using 19653-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 19653-1-AP (PPP3CC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using PPP3CC Polyclonal antibody (19653-1-AP)

    WB analysis of human testis using 19653-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 19653-1-AP (PPP3CC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using PPP3CC Polyclonal antibody (19653-1-AP)

    WB analysis of mouse skeletal muscle using 19653-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19653-1-AP (PPP3CC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using PPP3CC Polyclonal antibody (19653-1-AP)

    WB analysis of Jurkat using 19653-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19653-1-AP (PPP3CC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using PPP3CC Polyclonal antibody (19653-1-AP)

    IHC staining of human stomach using 19653-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 19653-1-AP (PPP3CC antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse testis tissue, human testis tissue, Jurkat cells, MCF-7 cells, mouse brain tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

19653-1-AP targets PPP3CC in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP3CC fusion protein Ag7619
Full Name protein phosphatase 3 (formerly 2B), catalytic subunit, gamma isoform
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC004864
Gene Symbol PPP3CC
Gene ID (NCBI) 5533
RRIDAB_10695630
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PPP3CC antibody 19653-1-APDownload protocol
IHC protocol for PPP3CC antibody 19653-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

FASEB J

Roles of glycogen synthase kinase 3 alpha and calcineurin in regulating the ability of sperm to fertilize eggs.

Authors - Souvik Dey
View Article
humanWB

BMC Complement Med Ther

Shikonin reactivates TSGs GADD45B and PPP3CC to block NSCLC cell proliferation and migration through JNK/P38/MAPK signaling pathways

Authors - Yujia Zhao
View Article