- Featured Product
- KD/KO Validated
PPP3CB-specific Polyclonal antibody
PPP3CB-specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 55148-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | NIH/3T3 cells, mouse brain tissue, fetal human brain tissue, rat brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human heart tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A431 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 4 publications below |
IHC | See 2 publications below |
IF | See 1 publications below |
Product Information
55148-1-AP targets PPP3CB-specific in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform |
Calculated Molecular Weight | 59 kDa |
Observed Molecular Weight | 59 kDa |
GenBank Accession Number | NM_021132 |
Gene Symbol | PPP3CB |
Gene ID (NCBI) | 5532 |
RRID | AB_10859260 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PPP3CB is one of the three isoforms of the A subunit of calcineurin (CnA). Calcineurin is a calcium-dependent serine/threonine phosphatase. It consists of two subunits: subunit A (CnA) and subunit B (CnB). For CnA, three isoforms PPP3CA (CnAα), PPP3CB (CnAβ) and PPP3CC (CnAγ) have been identified. PPP3CB is a calcium-dependent, calmodulin-stimulated protein phosphatase. It may have a role in the calmodulin activation of calcineurin. This antibody is specific to PPP3CB.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for PPP3CB-specific antibody 55148-1-AP | Download protocol |
IHC protocol for PPP3CB-specific antibody 55148-1-AP | Download protocol |
IF protocol for PPP3CB-specific antibody 55148-1-AP | Download protocol |
IP protocol for PPP3CB-specific antibody 55148-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Clin Invest MicroRNA-30 family members regulate calcium/calcineurin signaling in podocytes. | ||
Mol Psychiatry A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling. | ||
J Mol Med (Berl) Angiotensin II induces calcium/calcineurin signaling and podocyte injury by downregulating microRNA-30 family members. | ||
Cancer Commun (Lond) HMMR alleviates endoplasmic reticulum stress by promoting autophagolysosomal activity during endoplasmic reticulum stress-driven hepatocellular carcinoma progression |