PPP2R3A Polyclonal antibody
PPP2R3A Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 14720-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | PC-3 cells, MCF-7 cells |
Positive IHC detected in | human skin tissue, human brain tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 2 publications below |
IF | See 1 publications below |
Product Information
14720-1-AP targets PPP2R3A in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PPP2R3A fusion protein Ag6450 |
Full Name | protein phosphatase 2 (formerly 2A), regulatory subunit B'', alpha |
Calculated Molecular Weight | 130 kDa |
Observed Molecular Weight | 130 kDa |
GenBank Accession Number | BC065531 |
Gene Symbol | PPP2R3A |
Gene ID (NCBI) | 5523 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PPP2R3A(Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit alpha), also named as PPP2R3, has 2 isoforms produced by alternative splicing named as PR130 and PR72. The protein contains stretches of basic residues that may be putative nuclear localization signals, and a cluster of proline residues in an N-terminal region common to both isoforms that the isoform PR130 but not PR72 is variably expressed in a number of tumor cell lines. The PR72 and PR130 cDNAs encode 72 and 130 kDa proteins when expressed in rabbit reticulocyte lysates.(PMID:8392071).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for PPP2R3A antibody 14720-1-AP | Download protocol |
IHC protocol for PPP2R3A antibody 14720-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Biosci GREM1/PPP2R3A expression in heterogeneous fibroblasts initiates pulmonary fibrosis. | ||
Cell Signal PP2A-AMPKα-HSF1 axis regulates metal-induced HSP expression and ROS clearance. |