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  • Featured Product
  • KD/KO Validated

PPP2R1A Polyclonal antibody

PPP2R1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 15882-1-AP

Synonyms

Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform, PR65A, PP2A subunit A isoform R1-alpha, PP2A subunit A isoform PR65-alpha, Medium tumor antigen-associated 61 kDa protein

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using PPP2R1A Polyclonal antibody (15882-1-AP)

    WB analysis using 15882-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15882-1-AP (PPP2R1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PPP2R1A Polyclonal antibody (15882-1-AP)

    WB analysis using 15882-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15882-1-AP (PPP2R1A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PPP2R1A Polyclonal antibody (15882-1-AP)

    WB analysis using 15882-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15882-1-AP (PPP2R1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using PPP2R1A Polyclonal antibody (15882-1-AP)

    WB analysis of mouse kidney using 15882-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15882-1-AP (PPP2R1A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SMMC-7721 cells using PPP2R1A Polyclonal antibody (15882-1-AP)

    WB analysis of SMMC-7721 using 15882-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 15882-1-AP (PPP2R1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using PPP2R1A Polyclonal antibody (15882-1-AP)

    IP experiment of HepG2 using 15882-1-AP

    IP result of anti-PPP2R1A (IP:15882-1-AP, 4ug; Detection:15882-1-AP 1:300) with HepG2 cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PPP2R1A Polyclonal antibody (15882-1-AP)

    IHC staining of human kidney using 15882-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15882-1-AP (PPP2R1A antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PPP2R1A Polyclonal antibody (15882-1-AP)

    IHC staining of human kidney using 15882-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15882-1-AP (PPP2R1A antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PPP2R1A Polyclonal antibody (15882-1-AP)

    IHC staining of mouse kidney using 15882-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15882-1-AP (PPP2R1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PPP2R1A Polyclonal antibody (15882-1-AP)

    IHC staining of mouse kidney using 15882-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15882-1-AP (PPP2R1A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PPP2R1A Polyclonal antibody (15882-1-AP)

    IHC staining of human ovary tumor using 15882-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15882-1-AP (PPP2R1A antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PPP2R1A Polyclonal antibody (15882-1-AP)

    IHC staining of human ovary tumor using 15882-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15882-1-AP (PPP2R1A antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using PPP2R1A Polyclonal antibody (15882-1-AP)

    IF Staining of HEK-293 using 15882-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PPP2R1A antibody (15882-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using PPP2R1A Polyclonal antibody (15882-1-AP)

    IF Staining of HEK-293 using 15882-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PPP2R1A antibody (15882-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Tested Applications

Positive WB detected inHepG2 cells, A431 cells, mouse kidney tissue, rat liver tissue, SMMC-7721 cells, mouse liver tissue, L02 cells
Positive IP detected inHepG2 cells
Positive IHC detected inmouse kidney tissue, human kidney tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15882-1-AP targets PPP2R1A in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP2R1A fusion protein Ag8661
Full Name protein phosphatase 2 (formerly 2A), regulatory subunit A, alpha isoform
Calculated Molecular Weight 589 aa, 65 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC001537
Gene Symbol PPP2R1A
Gene ID (NCBI) 5518
RRIDAB_2237574
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPP2R1A belongs to the phosphatase 2A regulatory subunit A family. PPP2R1A encodes the α-isoform of the scaffolding subunit of the serine/threonine protein phosphatase 2A (PP2A) holoenzyme. This tumour suppressor complex is involved in growth and survival pathways and PPP2R1A mutations in endometrial high-grade serous carcinomas are a frequent and potentially targetable feature of this disease(PMID: 21381030). The PPP2R1A gene has 86% identity with the PPP2R1B gene(PMID:7681822).

Protocols

Product Specific Protocols
WB protocol for PPP2R1A antibody 15882-1-APDownload protocol
IHC protocol for PPP2R1A antibody 15882-1-APDownload protocol
IF protocol for PPP2R1A antibody 15882-1-APDownload protocol
IP protocol for PPP2R1A antibody 15882-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Science

Identification of Integrator-PP2A complex (INTAC), an RNA polymerase II phosphatase.

Authors - Hai Zheng
View Article
humanWB

Mol Cell

SPT5 stabilizes RNA polymerase II, orchestrates transcription cycles, and maintains the enhancer landscape.

Authors - Shibin Hu
View Article
humanWB

Front Cell Dev Biol

BRG1 Activates PR65A Transcription to Regulate NO Bioavailability in Vascular Endothelial Cells.

Authors - Baoyu Chen
View Article
humanWB

Cancer Cell Int

PPP2R1A silencing suppresses LUAD progression by sensitizing cells to nelfinavir-induced apoptosis and pyroptosis

Authors - Yating Liu
  • KD Validated
View Article
human,pigWB,CoIP

Microbiol Spectr

TurboID Screening of the OmpP2 Protein Reveals Host Proteins Involved in Recognition and Phagocytosis of Glaesserella parasuis by iPAM Cells

Authors - Changsheng Jiang
  • KD Validated
View Article
humanCoIP

Dev Cell

Fam72a functions as a cell-cycle-controlled gene during proliferation and antagonizes apoptosis through reprogramming PP2A substrates

Authors - Yuan Fu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Wissem (Verified Customer) (07-30-2024)

antibody works too detect PPP2R1A in HEK293 and U2OS cell extracts

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
FH

Zee (Verified Customer) (01-28-2020)

It worked very well for western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse heart tissue