Various lysates were subjected to SDS PAGE followed by western blot with 10748-1-AP (DARPP32 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 10748-1-AP (DARPP32 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of mouse brain using 10748-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10748-1-AP (DARPP32 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10748-1-AP (DARPP32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 10748-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10748-1-AP (DARPP32 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10748-1-AP (DARPP32 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 10748-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10748-1-AP (DARPP32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10748-1-AP (DARPP32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 10748-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DARPP32/PPP1R1B antibody (10748-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DARPP32/PPP1R1B antibody (10748-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of SH-SY5Y using 10748-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using DARPP32/PPP1R1B antibody (10748-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using DARPP32/PPP1R1B antibody (10748-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected in
mouse brain tissue
Positive IF/ICC detected in
SH-SY5Y cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:2000
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)-P
IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Dopamine- and cAMP-regulated neuronal phosphoprotein (DARPP32) , also known as protein phosphatase 1 regulatory subunit 1B (PPP1R1B), is a substrate of cAMP-dependent protein kinase (PKA) enriched in dopamine-innervated brain areas. Overexpression of DARPP32 has been shown to block gefitinib-induced apoptosis in gastric tumors by promoting EGFR and ERBB3 heterodimerization (PMID: 21741919). DARPP32 also drives gefitinib-refractory lung tumor growth through similar ERBB3-mediated resistance mechanisms(PMID: 34675407). The molecular weight of DARPP32 is about 32 kDa.
Ginsenoside Rg1 exerts neuroprotective effects in 3-nitropronpionic acid-induced mouse model of Huntington's disease via suppressing MAPKs and NF-κB pathways in the striatum.
Disruption of Cdk5-GluN2B complex by a small interfering peptide attenuates social isolation-induced escalated intermale attack behavior and hippocampal oxidative stress in mice
Various lysates were subjected to SDS PAGE followed by western blot with 10748-1-AP (DARPP32 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse brain using 10748-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10748-1-AP (DARPP32 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 10748-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10748-1-AP (DARPP32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 10748-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10748-1-AP (DARPP32 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 10748-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10748-1-AP (DARPP32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P Figures
IF Staining of mouse brain using 10748-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DARPP32/PPP1R1B antibody (10748-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF/ICC Figures
IF Staining of SH-SY5Y using 10748-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using DARPP32/PPP1R1B antibody (10748-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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