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PPP1R10 Polyclonal antibody

PPP1R10 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24450-1-AP

Synonyms

CAT53, FB19, p99, PNUTS, PP1 binding protein of 114 kDa, PP1R10, PPP1R10, Protein FB19

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using PPP1R10 Polyclonal antibody (24450-1-AP)

    WB analysis of HepG2 using 24450-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 24450-1-AP (PPP1R10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PPP1R10 Polyclonal antibody (24450-1-AP)

    IF Staining of HepG2 using 24450-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 24450-1-AP (PPP1R10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24450-1-AP is PPP1R10 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP1R10 fusion protein Ag20091
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 10
Calculated Molecular Weight 940 aa, 99 kDa
Observed Molecular Weight 99 kDa
GenBank Accession NumberBC150310
Gene Symbol PPP1R10
Gene ID (NCBI) 5514
RRIDAB_2879554
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PPP1R10 antibody 24450-1-APDownload protocol
IF protocol for PPP1R10 antibody 24450-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Nat Cell Biol

A regulated PNUTS mRNA to lncRNA splice switch mediates EMT and tumour progression.

Authors - Simon Grelet
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Roy (Verified Customer) (06-15-2024)

Perfect to detect PNUTS by WB (1/1000, Overnight incubation at 4°C) and by IF (1/250) in HeLa cells

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HeLa
FH

Fabienne (Verified Customer) (06-11-2024)

Works well in western blot

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa cell line