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LRR 1 Polyclonal antibody

LRR 1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11628-1-AP

Synonyms

4 1BBLRR, LRR 1, LRR repeat protein 1, LRR1, PPIL5

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of U-937 cells using LRR 1 Polyclonal antibody (11628-1-AP)

    WB analysis of U-937 using 11628-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 11628-1-AP (LRR 1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using LRR 1 Polyclonal antibody (11628-1-AP)

    IHC staining of human pancreas cancer using 11628-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11628-1-AP (LRR 1 antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inU-937 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11628-1-AP targets LRR 1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LRR 1 fusion protein Ag2159
Full Name peptidylprolyl isomerase (cyclophilin)-like 5
Calculated Molecular Weight 46.7 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC030142
Gene Symbol LRR 1
Gene ID (NCBI) 122769
RRIDAB_2169750
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LRR 1 antibody 11628-1-APDownload protocol
IHC protocol for LRR 1 antibody 11628-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Exp Clin Cancer Res

HJURP promotes hepatocellular carcinoma proliferation by destabilizing p21 via the MAPK/ERK1/2 and AKT/GSK3β signaling pathways.

Authors - Tianchi Chen
View Article
humanWB

Immunology

Tumour YAP1 and PTEN expression correlates with tumour-associated myeloid suppressor cell expansion and reduced survival in colorectal cancer.

Authors - Rong Yang
View Article
ratWB,IF

J Immunol Res

The Protective Effect of Low Dose of Lipopolysaccharide Pretreatment on Endotoxin-Induced Uveitis in Rats Is Associated with Downregulation of CSF-1 and Upregulation of LRR-1.

Authors - Yunli Ling
View Article