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Cyclophilin B Polyclonal antibody

Name: Cyclophilin B Polyclonal antibody
Brand: Proteintech
SKU: 11607-1-AP
Rating: 5.00 (2 reviews)

Cyclophilin B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Reviews (2)

Cat no : 11607-1-AP

Synonyms

PPIB, PPIase B, Peptidyl-prolyl cis-trans isomerase B, EC:5.2.1.8, CYP-S1

Validation Data Gallery

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AllWBIHCIF/ICC

WB analysis of HeLa using 11607-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11607-1-AP (Cyclophilin B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11607-1-AP
WB result of Cyclophilin B antibody (11607-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclophilin B transfected HeLa cells.
WB analysis of Raji using 11607-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 11607-1-AP (Cyclophilin B antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of U-937 using 11607-1-AP
U-937 cells were subjected to SDS PAGE followed by western blot with 11607-1-AP (Cyclophilin B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11607-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 11607-1-AP (Cyclophilin B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 11607-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 11607-1-AP (Cyclophilin B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of U-937 using 11607-1-AP
U-937 cells were subjected to SDS PAGE followed by western blot with 11607-1-AP (Cyclophilin B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 11607-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11607-1-AP (Cyclophilin B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human lymphoma using 11607-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11607-1-AP (Cyclophilin B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 11607-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11607-1-AP (Cyclophilin B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human normal colon using 11607-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11607-1-AP (Cyclophilin B antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human normal colon using 11607-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11607-1-AP (Cyclophilin B antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 11607-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11607-1-AP (Cyclophilin B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 11607-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11607-1-AP (Cyclophilin B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 11607-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11607-1-AP (Cyclophilin B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 11607-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11607-1-AP (Cyclophilin B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 11607-1-AP
Immunofluorescent analysis of HeLa cells using 11607-1-AP (Cyclophilin B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 11607-1-AP

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Tested Applications

Positive WB detected in	HeLa cells, U-937 cells, Raji cells, HepG2 cells, mouse liver tissue, NIH/3T3 cells, Jurkat cells, RAW 264.7 cells, rat liver tissue
Positive IHC detected in	human normal colon, human liver cancer tissue, human lymphoma tissue, human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:300-1:1200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 13 publications below
IHC	See 3 publications below
IF	See 2 publications below

Product Information

11607-1-AP targets Cyclophilin B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Cyclophilin B fusion protein Ag2161
Full Name	peptidylprolyl isomerase B (cyclophilin B)
Calculated Molecular Weight	216 aa, 24 kDa
Observed Molecular Weight	19-24 kDa
GenBank Accession Number	BC032138
Gene Symbol	Cyclophilin B
Gene ID (NCBI)	5479
RRID	AB_2237519
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPIB, also named as CYPB, SCYLP, PPIase B and CYP-S1, belongs to the cyclophilin-type PPIase family and PPIase B subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes. The full-length of PPIB has 216aa (24 kDa), and a 33aa-signal peptide could be removed in the matrue form (about 19 kDa).

Protocols

Product Specific Protocols
WB protocol for Cyclophilin B antibody 11607-1-AP	Download protocol
IHC protocol for Cyclophilin B antibody 11607-1-AP	Download protocol
IF protocol for Cyclophilin B antibody 11607-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cell Metabolic Diversity in Human Non-Small Cell Lung Cancer Cells. Authors - Pei-Hsuan Chen View Article
human	WB,IHC	Am J Hum Genet PPIB mutations cause severe osteogenesis imperfecta. Authors - van Dijk Fleur S FS View Article
mouse	WB	Cell Prolif OGG1 aggravates renal ischemia-reperfusion injury by repressing PINK1-mediated mitophagy Authors - Fan Zhao View Article
human	WB,IF	Hum Mol Genet Mutations in PPIB (cyclophilin B) delay type I procollagen chain association and result in perinatal lethal to moderate osteogenesis imperfecta phenotypes. Authors - Pyott Shawna M SM View Article
mouse	WB	Cells HDAC6 Inhibition Alleviates Ischemia- and Cisplatin-Induced Acute Kidney Injury by Promoting Autophagy Authors - Lang Shi View Article
human,mouse	WB,IF	J Virol CD147/EMMPRIN acts as a functional entry receptor for measles virus on epithelial cells. Authors - Watanabe Akira A View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Ganesh (Verified Customer) (02-07-2024)

Whole cell extracts of HAP1 (human) cells were resolved on a 4-12% Bis-Tris Gel and proteins were transferred onto a nitrocellulose membrane. Membrane was incubated overnight at 4 deg C with cyclophilin antibody (diluted in 5% Milk in TBS-T). I used this antibody as a loading control in this experiment. The signal was very quick on a film using regular ECL substrate.

Applications: Western Blot
Primary Antibody Dilution: 1:5000 dilution
Cell Tissue Type: HAP1

Cyclophilin B Antibody Western Blot validation (1:5000 dilution dilution) in HAP1 (Cat no:11607-1-AP)

Parijat (Verified Customer) (08-21-2023)

Works well.

Applications: Western Blot
Primary Antibody Dilution: 1:5000

Cyclophilin B Polyclonal antibody

Cyclophilin B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of HeLa using 11607-1-AP

WB analysis of HeLa using 11607-1-AP

WB analysis of Raji using 11607-1-AP

WB analysis of U-937 using 11607-1-AP

WB analysis of HepG2 using 11607-1-AP

WB analysis of mouse liver using 11607-1-AP

WB analysis of U-937 using 11607-1-AP

WB analysis of NIH/3T3 using 11607-1-AP

IHC staining of human lymphoma using 11607-1-AP

IHC staining of human lymphoma using 11607-1-AP

IHC staining of human normal colon using 11607-1-AP

IHC staining of human normal colon using 11607-1-AP

IHC staining of human liver cancer using 11607-1-AP

IHC staining of human liver cancer using 11607-1-AP

IHC staining of human pancreas cancer using 11607-1-AP

IHC staining of human pancreas cancer using 11607-1-AP

IF Staining of HeLa using 11607-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Metabolic Diversity in Human Non-Small Cell Lung Cancer Cells.

PPIB mutations cause severe osteogenesis imperfecta.

OGG1 aggravates renal ischemia-reperfusion injury by repressing PINK1-mediated mitophagy

Mutations in PPIB (cyclophilin B) delay type I procollagen chain association and result in perinatal lethal to moderate osteogenesis imperfecta phenotypes.

HDAC6 Inhibition Alleviates Ischemia- and Cisplatin-Induced Acute Kidney Injury by Promoting Autophagy

CD147/EMMPRIN acts as a functional entry receptor for measles virus on epithelial cells.

Reviews