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POU5F2 Polyclonal antibody

POU5F2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26362-1-AP

Synonyms

POU5F2, SPRM 1, SPRM1

Validation Data Gallery

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  • Western Blot (WB) analysis of NCCIT cells using POU5F2 Polyclonal antibody (26362-1-AP)

    WB analysis of NCCIT using 26362-1-AP

    NCCIT cell were subjected to SDS PAGE followed by western blot with 26362-1-AP (POU5F2 Antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NCCIT cells using POU5F2 Polyclonal antibody (26362-1-AP)

    WB analysis of NCCIT using 26362-1-AP

    NCCIT cell were subjected to SDS PAGE followed by western blot with 26362-1-AP (POU5F2 Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inNCCIT cell

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26362-1-AP is POU5F2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POU5F2 fusion protein Ag23803
Full Name POU domain class 5, transcription factor 2
Observed Molecular Weight 42-45 kDa
GenBank Accession NumberBC029532
Gene Symbol POU5F2
Gene ID (NCBI) 134187
RRIDAB_2880491
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POU5F2, also named as POU domain, class 5, transcription factor 2m, is a 328 amino acid protein, which contains 1 homeobox DNA-binding domain and 1 POU-specific domain and belongs to the POU transcription factor family. POU5F2 as a transcription factor, localizes in the nuclues and may exert a regulatory function in meiotic events that are required for terminal differentiation of male germ cell.

Protocols

Product Specific Protocols
WB protocol for POU5F2 antibody 26362-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols