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POU3F4 Polyclonal antibody

POU3F4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 25114-1-AP

Synonyms

BRAIN 4, Brn 4, BRN4, DFN3, DFNX2, Oct 9, Octamer binding protein 9, OTF 9, OTF9, POU class 3 homeobox 4, POU3F4

Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using POU3F4 Polyclonal antibody (25114-1-AP)

    WB analysis of fetal human brain using 25114-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 25114-1-AP (POU3F4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected infetal human brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

The immunogen of 25114-1-AP is POU3F4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POU3F4 fusion protein Ag18925
Full Name POU class 3 homeobox 4
Calculated Molecular Weight 361 aa, 39 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC146551
Gene Symbol POU3F4
Gene ID (NCBI) 5456
RRIDAB_2879903
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OTF9 also named as BRN4 and POU3F4, belongs to the POU transcription factor family and Class-3 subfamily. OTF9 is a transcription factor which exerts its primary action widely during early neural development and in a very limited set of neurons in the mature brain. Mutation of OTF9 will cause of X-linked deafness type 3 (DFN3).

Protocols

Product Specific Protocols
WB protocol for POU3F4 antibody 25114-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

J Biol Eng

An engineered ligand-responsive Csy4 endoribonuclease controls transgene expression from Sendai virus vectors

Authors - Takumi Kishimoto
View Article