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POTEA-Specific Polyclonal antibody

POTEA-Specific Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20203-1-AP

Synonyms

A26A1, ANKRD26 like family A member 1, POTE 8, POTE8, POTEA, POTEA-Specific

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse ovary tissue using POTEA-Specific Polyclonal antibody (20203-1-AP)

    IHC staining of mouse ovary using 20203-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 20203-1-AP (POTEA-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tissue using POTEA-Specific Polyclonal antibody (20203-1-AP)

    IHC staining of human ovary using 20203-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 20203-1-AP (POTEA-Specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using POTEA-Specific Polyclonal antibody (20203-1-AP)

    IHC staining of human ovary using 20203-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 20203-1-AP (POTEA-Specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using POTEA-Specific Polyclonal antibody (20203-1-AP)

    IF Staining of HepG2 using 20203-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using POTEA-Specific antibody (20203-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inmouse ovary tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20203-1-AP targets POTEA-Specific in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name POTE ankyrin domain family, member A
Calculated Molecular Weight 56 kDa
GenBank Accession NumberNM_001002920
Gene Symbol POTEA
Gene ID (NCBI) 340441
RRIDAB_10666735
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POTEA, also named as A26A1 and POTE8, belongs to the POTE family. POTE has an important signaling function in the reproductive system. The antibody is specific to POTEA.

Protocols

Product Specific Protocols
IHC protocol for POTEA-Specific antibody 20203-1-APDownload protocol
IF protocol for POTEA-Specific antibody 20203-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols