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POTEA Polyclonal antibody

POTEA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24593-1-AP

Synonyms

POTE-8, POTE8, POTE 8, ANKRD26-like family A member 1, ANKRD26 like family A member 1

Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using POTEA Polyclonal antibody (24593-1-AP)

    WB analysis of human testis using 24593-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 24593-1-AP (POTEA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using POTEA Polyclonal antibody (24593-1-AP)

    WB analysis of mouse skeletal muscle using 24593-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 24593-1-AP (POTEA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using POTEA Polyclonal antibody (24593-1-AP)

    IHC staining of human ovary cancer using 24593-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 24593-1-AP (POTEA antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using POTEA Polyclonal antibody (24593-1-AP)

    IHC staining of human ovary cancer using 24593-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 24593-1-AP (POTEA antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tissue using POTEA Polyclonal antibody (24593-1-AP)

    IHC staining of human ovary using 24593-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 24593-1-AP (POTEA Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using POTEA Polyclonal antibody (24593-1-AP)

    IHC staining of human testis using 24593-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24593-1-AP (POTEA Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using POTEA Polyclonal antibody (24593-1-AP)

    IHC staining of human testis using 24593-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24593-1-AP (POTEA Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inhuman testis tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman ovary cancer tissue, human ovary tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24593-1-AP targets POTEA in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POTEA fusion protein Ag20228
Full Name POTE ankyrin domain family, member A
Calculated Molecular Weight 498 aa, 56 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC153076
Gene Symbol POTEA
Gene ID (NCBI) 340441
RRIDAB_2879627
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POTEA also named as ANKRD26 like family A member 1 or POTE8 is a 498 amino acid protein, which contains five ANK repeats and belongs to the POTE family. POTEA has an important signaling function in the reproductive system.

Protocols

Product Specific Protocols
WB protocol for POTEA antibody 24593-1-APDownload protocol
IHC protocol for POTEA antibody 24593-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols