PON1 Polyclonal antibody
PON1 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 18155-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human plasma, mouse liver tissue |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 3 publications below |
IHC | See 2 publications below |
IF | See 2 publications below |
IP | See 1 publications below |
ELISA | See 1 publications below |
Product Information
18155-1-AP targets PON1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PON1 fusion protein Ag12934 |
Full Name | paraoxonase 1 |
Calculated Molecular Weight | 355 aa, 40 kDa |
Observed Molecular Weight | 35 kDa, 40-45 kDa |
GenBank Accession Number | BC074719 |
Gene Symbol | PON1 |
Gene ID (NCBI) | 5444 |
RRID | AB_2166315 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PON1, also named PON and K-45, belongs to the paraoxonase family. PON1 hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. It is capable of hydrolyzing a broad spectrum of organophosphate substrates and several aromatic carboxylic acid esters. PON1 may mediate enzymatic protection of low-density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. PON1 was identified as a triple band in a range of approximately 35-40 kDa. For glycosylated, the MW of PON1 is migrated 42-45kd, while the 35 kDa band represents the unglycosylated form (PMID: 17906223, 32002976, 21852972). The antibody has cross-reaction to PON2 and PON3.
Protocols
Product Specific Protocols | |
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WB protocol for PON1 antibody 18155-1-AP | Download protocol |
IHC protocol for PON1 antibody 18155-1-AP | Download protocol |
IP protocol for PON1 antibody 18155-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Death Dis GPx1 is involved in the induction of protective autophagy in pancreatic cancer cells in response to glucose deprivation. | ||
Cell Mol Neurobiol PGC-1α Inhibits Schwann Cell Dedifferentiation and Delays Peripheral Nerve Degeneration by Targeting PON1 | ||
Biomed Pharmacother GYY4137, a H2S donor, ameliorates kidney injuries in diabetic mice by modifying renal ROS-associated enzymes | ||
Biochim Biophys Acta Mol Cell Biol Lipids Paraoxonase 1 in endothelial cells impairs vasodilation induced by arachidonic acid lactone metabolite. |