PON1 Polyclonal antibody

PON1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18155-1-AP

Synonyms

A esterase 1, Aromatic esterase 1, ESA, K 45, paraoxonase 1, PON, PON 1, PON1, Serum aryldialkylphosphatase 1



Tested Applications

Positive WB detected inhuman plasma, mouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18155-1-AP targets PON1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PON1 fusion protein Ag12934
Full Name paraoxonase 1
Calculated Molecular Weight 355 aa, 40 kDa
Observed Molecular Weight35 kDa, 40-45 kDa
GenBank Accession NumberBC074719
Gene Symbol PON1
Gene ID (NCBI) 5444
RRIDAB_2166315
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PON1, also named PON and K-45, belongs to the paraoxonase family. PON1 hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. It is capable of hydrolyzing a broad spectrum of organophosphate substrates and several aromatic carboxylic acid esters. PON1 may mediate enzymatic protection of low-density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. PON1 was identified as a triple band in a range of approximately 35-40 kDa. For glycosylated, the MW of PON1 is migrated 42-45kd, while the 35 kDa band represents the unglycosylated form (PMID: 17906223, 32002976, 21852972). The antibody has cross-reaction to PON2 and PON3.

Protocols

Product Specific Protocols
WB protocol for PON1 antibody 18155-1-APDownload protocol
IHC protocol for PON1 antibody 18155-1-APDownload protocol
IP protocol for PON1 antibody 18155-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

GPx1 is involved in the induction of protective autophagy in pancreatic cancer cells in response to glucose deprivation.

Authors - Qingcai Meng
humanIF

Front Cell Dev Biol

Significance of Liver Zonation in Hepatocellular Carcinoma.

Authors - Shizhe Yu
mouseWB,IP

Cell Mol Neurobiol

PGC-1α Inhibits Schwann Cell Dedifferentiation and Delays Peripheral Nerve Degeneration by Targeting PON1

Authors - Ying Zou
mouseWB,IF

Biomed Pharmacother

GYY4137, a H2S donor, ameliorates kidney injuries in diabetic mice by modifying renal ROS-associated enzymes

Authors - Xueqi Chen
human,mouseIHC,ELISA

Elife

Multi-targeted therapy resistance via drug-induced secretome fucosylation

Authors - Mark Borris D Aldonza
mouseIHC

Biochim Biophys Acta Mol Cell Biol Lipids

Paraoxonase 1 in endothelial cells impairs vasodilation induced by arachidonic acid lactone metabolite.

Authors - Dan Gilad