- Featured Product
- KD/KO Validated
POM121 Polyclonal antibody
POM121 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
Cat no : 15645-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 6 publications below |
Product Information
15645-1-AP targets POM121 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | POM121 fusion protein Ag8177 |
| Full Name | POM121 membrane glycoprotein (rat) |
| Calculated Molecular Weight | 984 aa, 99 kDa |
| Observed Molecular Weight | 140-150 kDa |
| GenBank Accession Number | BC008794 |
| Gene Symbol | POM121 |
| Gene ID (NCBI) | 9883 |
| RRID | AB_2878160 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
POM121 is an integral membrane protein that localizes to the central spoke ring complex and participates in anchoring the nuclear pore complex to the nuclear envelope. Catalog#15645-1-AP do well in identifying the nuclear envelope in immunofluorescence experiments.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for POM121 antibody 15645-1-AP | Download protocol |
| IHC protocol for POM121 antibody 15645-1-AP | Download protocol |
| IF protocol for POM121 antibody 15645-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Neurosci Interactions between ALS-linked FUS and nucleoporins are associated with defects in the nucleocytoplasmic transport pathway. | ||
Elife Nuclear pore heterogeneity influences HIV-1 infection and the antiviral activity of MX2. | ||
Cancer Biomark POM121 promotes proliferation and metastasis in non-small-cell lung cancer through TGF-β/SMAD and PI3K/AKT pathways
| ||
bioRxiv Altered nuclear envelope homeostasis is a key pathogenic event in C9ORF72-linked ALS/FTD |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH bing (Verified Customer) (08-06-2019) | Works great in Western Blot, I use it for Hela and Vero cells lysate, go dilution 1:5000 in 5% milk.
|
FH Yen-Chen (Verified Customer) (12-03-2018) |
|
