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  • Featured Product
  • KD/KO Validated

POLR2J Polyclonal antibody

POLR2J Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16403-1-AP

Synonyms

hRPB14, POLR2J, POLR2J1, RPB11, RPB11A, RPB11m

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using POLR2J Polyclonal antibody (16403-1-AP)

    WB analysis using 16403-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16403-1-AP (POLR2J antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using POLR2J Polyclonal antibody (16403-1-AP)

    WB analysis of human brain using 16403-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16403-1-AP (POLR2J antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A549 cells using POLR2J Polyclonal antibody (16403-1-AP)

    IP experiment of A549 using 16403-1-AP

    IP result of anti-POLR2J (IP:16403-1-AP, 4ug; Detection:16403-1-AP 1:500) with A549 cells lysate 2600 ug.

  • Immunohistochemistry (IHC) staining of human heart tissue using POLR2J Polyclonal antibody (16403-1-AP)

    IHC staining of human heart using 16403-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16403-1-AP (POLR2J antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using POLR2J Polyclonal antibody (16403-1-AP)

    IHC staining of human heart using 16403-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16403-1-AP (POLR2J antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using POLR2J Polyclonal antibody (16403-1-AP)

    IF Staining of A549 using 16403-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 16403-1-AP (POLR2J antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, human brain tissue, HeLa cells, MCF-7 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16403-1-AP targets POLR2J in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLR2J fusion protein Ag9520
Full Name polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa
Calculated Molecular Weight 115 aa, 13 kDa
Observed Molecular Weight 13 kDa
GenBank Accession NumberBC024165
Gene Symbol POLR2J
Gene ID (NCBI) 5439
RRIDAB_10666162
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for POLR2J antibody 16403-1-APDownload protocol
IHC protocol for POLR2J antibody 16403-1-APDownload protocol
IF protocol for POLR2J antibody 16403-1-APDownload protocol
IP protocol for POLR2J antibody 16403-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell

RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits

Authors - Yuanjun Li
View Article
mouseWB

Mol Cell

Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity

Authors - Yuanjun Li
View Article
mouseWB

Cell Biosci

Replication and transcription machinery for ranaviruses: components, correlation, and functional architecture.

Authors - Fei Ke
  • KD Validated
View Article
mouse

Genome Biol

Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture

Authors - Yongpeng Jiang
View Article