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Tested Applications
| Positive WB detected in | A549 cells, human brain tissue, HeLa cells, MCF-7 cells |
| Positive IP detected in | A549 cells |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
Product Information
16403-1-AP targets POLR2J in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | POLR2J fusion protein Ag9520 Predict reactive species |
| Full Name | polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa |
| Calculated Molecular Weight | 115 aa, 13 kDa |
| Observed Molecular Weight | 13 kDa |
| GenBank Accession Number | BC024165 |
| Gene Symbol | POLR2J |
| Gene ID (NCBI) | 5439 |
| RRID | AB_10666162 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P52435 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for POLR2J antibody 16403-1-AP | Download protocol |
| IHC protocol for POLR2J antibody 16403-1-AP | Download protocol |
| IF protocol for POLR2J antibody 16403-1-AP | Download protocol |
| IP protocol for POLR2J antibody 16403-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits | ||
Mol Cell Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity | ||
Cell Biosci Replication and transcription machinery for ranaviruses: components, correlation, and functional architecture.
| ||
Genome Biol Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture |
