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PNMAL1 Polyclonal antibody

PNMAL1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16965-1-AP

Synonyms

PNMA-like protein 1, PNMA8A, PNMA like protein 1, PNMA like 1, Paraneoplastic antigen-like protein 8A

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using PNMAL1 Polyclonal antibody (16965-1-AP)

    WB analysis of HEK-293 using 16965-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16965-1-AP (PNMAL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A2780 cells using PNMAL1 Polyclonal antibody (16965-1-AP)

    WB analysis of A2780 using 16965-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 16965-1-AP (PNMAL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PNMAL1 Polyclonal antibody (16965-1-AP)

    IF Staining of HepG2 using 16965-1-AP

    Immunofluorescent analysis of HepG2 cells, using PNMAL1 antibody 16965-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHEK-293 cells, A2780 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16965-1-AP targets PNMAL1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PNMAL1 fusion protein Ag10698
Full Name PNMA-like 1
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC026026
Gene Symbol PNMAL1
Gene ID (NCBI) 55228
RRIDAB_2167639
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PNMAL1 antibody 16965-1-APDownload protocol
IF protocol for PNMAL1 antibody 16965-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols