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PLCB3 Polyclonal antibody

PLCB3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 51139-1-AP

Synonyms

Phospholipase C beta 3, PLC beta 3, PLCB3

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PLCB3 Polyclonal antibody (51139-1-AP)

    WB analysis of HeLa using 51139-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 51139-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293T cells using PLCB3 Polyclonal antibody (51139-1-AP)

    WB analysis of HEK-293T using 51139-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 51139-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using PLCB3 Polyclonal antibody (51139-1-AP)

    WB analysis of MCF-7 using 51139-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 51139-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, HEK-293T cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

51139-1-AP targets PLCB3 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name phospholipase C, beta 3 (phosphatidylinositol-specific)
Calculated Molecular Weight 1234 aa, 139 kDa
Observed Molecular Weight 139 kDa
GenBank Accession NumberBC142681
Gene Symbol PLCB3
Gene ID (NCBI) 5331
RRIDAB_2881250
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PLCB3 antibody 51139-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols