Polycystin 2 Polyclonal antibody

Polycystin 2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 19126-1-AP

Synonyms

PKD2, Polycystwin, Polycystin-2, PKD4, PC2



Tested Applications

Positive WB detected inmouse kidney tissue, HEK-293 cells, human kidney tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19126-1-AP targets Polycystin 2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity human, mouse, rat, canine
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name polycystic kidney disease 2 (autosomal dominant)
Calculated Molecular Weight 110 kDa
Observed Molecular Weight 109 kDa
GenBank Accession NumberNM_000297
Gene Symbol PKD2
Gene ID (NCBI) 5311
RRIDAB_10641050
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Polycystin 2 (PKD2), the product of the gene mutated in type 2 autosomal dominant polycystic kidney disease, belongs to the polycystin family. PKD2 is a ~110-kDa six-transmembrane channel protein with cytoplasmic N- and C-termini. This protein functions as a Ca2+-activated intracellular Ca2+ release channel in the endoplasmic reticulum. It is also present in the plasma membrane, where it functions as a nonselective cation channel. In addition, PKD2 expression has been documented in the primary cilium of kidney epithelial cells, where it is believed to have an essential role in mediating Ca2+ entry in response to flow rate changes, suggesting that it may be part of a mechanosensing machinery residing in the primary cilium. (PMID: 16135816; 10497221)

Protocols

Product Specific Protocols
WB protocol for Polycystin 2 antibody 19126-1-APDownload protocol
IHC protocol for Polycystin 2 antibody 19126-1-APDownload protocol
IF protocol for Polycystin 2 antibody 19126-1-APDownload protocol
IP protocol for Polycystin 2 antibody 19126-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Mol Biosci

The Role of TRPC6 in Renal Ischemia/Reperfusion and Cellular Hypoxia/Reoxygenation Injuries.

Authors - Xin Hou
humanIHC

Hum Pathol

Down-regulation of polycystin in lymphatic malformations: Possible role in the proliferation of lymphatic endothelial cells.

Authors - Jian-Gang Ren
ratWB

Cell Physiol Biochem

iTRAQ-Based Proteomic Analysis of Neonatal Kidney from Offspring of Protein Restricted Rats Reveals Abnormalities in Intraflagellar Transport Proteins.

Authors - Xiaomei Liu
mouseIF,IHC,WB

Graefes Arch Clin Exp Ophthalmol

TRPP2 is located in the primary cilia of human non-pigmented ciliary epithelial cells

Authors - Wenxu Zheng
humanWB

Autophagy Rep

Shear stress induces autophagy in Schlemm's canal cells via primary cilia-mediated SMAD2/3 signaling pathway

Authors - Myoung Sup Shim
humanIF

J Am Soc Nephrol

Pkd2 Deficiency in Embryonic Aqp2 + Progenitor Cells Is Sufficient to Cause Severe Polycystic Kidney Disease

Authors - Akaki Tsilosani