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PIGB Polyclonal antibody

PIGB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12612-1-AP

Synonyms

GPI mannosyltransferase 3, GPI mannosyltransferase III, GPI MT III, PIG B, PIGB

Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using PIGB Polyclonal antibody (12612-1-AP)

    WB analysis of human liver using 12612-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 12612-1-AP (PIGB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using PIGB Polyclonal antibody (12612-1-AP)

    WB analysis of Raji using 12612-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 12612-1-AP (PIGB antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using PIGB Polyclonal antibody (12612-1-AP)

    WB analysis of mouse testis using 12612-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12612-1-AP (PIGB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using PIGB Polyclonal antibody (12612-1-AP)

    WB analysis of human brain using 12612-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12612-1-AP (PIGB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PIGB Polyclonal antibody (12612-1-AP)

    IHC staining of human prostate cancer using 12612-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12612-1-AP (PIGB antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inhuman liver tissue, human brain tissue, mouse testis tissue, Raji cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12612-1-AP targets PIGB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIGB fusion protein Ag3244
Full Name phosphatidylinositol glycan anchor biosynthesis, class B
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC017711
Gene Symbol PIGB
Gene ID (NCBI) 9488
RRIDAB_2164664
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PIGB antibody 12612-1-APDownload protocol
IHC protocol for PIGB antibody 12612-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem J

Accelerated urokinase-receptor protein turnover triggered by interference with the addition of the glycolipid anchor.

Authors - Avila Hector H
View Article